Anticancer potential of exosome-like nanoparticles isolated from Acorus calamus in breast cancer.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-24 DOI:10.1007/s13205-025-04349-8

Sunny Gupta, Shipra Gupta, Manju Singh, Ashok Kumar Patel

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引用次数: 0

Abstract

Plant-derived exosome-like nanoparticles represent a novel class of plant-based therapeutics with potential anti-cancer applications. The present study aims to isolate, characterize, and evaluate the Acorus calamus-derived exosome-like nanoparticles (ACENPs) for their cytotoxic and apoptotic effects on breast cancer cells. Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) revealed that ACENPs exhibited a mean hydrodynamic diameter of 122.4 ± 5.0 nm and a particle concentration of 1.58 × 10¹¹ particles/mL. Cellular uptake studies confirmed the efficient internalization of ACENPs in MCF-7, MDA-MB-453, and MDA-MB-231 breast cancer cells. Cytotoxicity assay demonstrated a significant reduction in cell viability by 17.6%, 25%, and 35.8% in MCF-7, MDA-MB-231, and MDA-MB-453 breast cancer cells, respectively. Apoptosis induction was validated through AO/EB staining, DAPI nuclear fragmentation assays, annexin V-FITC/PI staining, and Western blot analysis of apoptosis-related proteins. Treatment with ACENPs resulted in an increased Bax/Bcl-2 ratio, indicating apoptotic activation. Metabolomic profiling identified bioactive compounds such as arecoline, trigonelline, asarone, and gingerol, known for their anti-cancer properties. Our study findings highlight that ACENPs could be utilized as a promising therapeutic approach for breast cancer treatment. We propose to conduct future research focusing on in vivo validation and optimizing large-scale production for clinical translation.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04349-8.

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菖蒲外泌体样纳米颗粒对乳腺癌的抗癌作用。

植物衍生的外泌体样纳米颗粒代表了一类具有潜在抗癌应用的新型植物治疗药物。本研究旨在分离、表征和评价菖蒲衍生的外泌体样纳米颗粒（ACENPs）对乳腺癌细胞的细胞毒性和凋亡作用。纳米颗粒跟踪分析（NTA）和透射电子显微镜（TEM）显示，ACENPs的平均水动力直径为122.4±5.0 nm，颗粒浓度为1.58 × 1011个/mL。细胞摄取研究证实了MCF-7、MDA-MB-453和MDA-MB-231乳腺癌细胞中ACENPs的有效内化。细胞毒性试验表明，MCF-7、MDA-MB-231和MDA-MB-453乳腺癌细胞的细胞活力分别显著降低17.6%、25%和35.8%。通过AO/EB染色、DAPI核片段化、膜联蛋白V-FITC/PI染色和凋亡相关蛋白的Western blot分析证实凋亡诱导作用。ACENPs导致Bax/Bcl-2比值升高，表明凋亡活化。代谢组学分析鉴定出生物活性化合物，如槟榔碱、葫芦巴碱、细辛酮和姜辣素，以其抗癌特性而闻名。我们的研究结果强调，ACENPs可以作为一种有前途的治疗乳腺癌的方法。我们建议将未来的研究重点放在体内验证和优化临床翻译的大规模生产上。补充信息：在线版本包含补充资料，下载地址：10.1007/s13205-025-04349-8。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.