Antimicrobial and Anticancer Potential of Chitinophaga sp. S167 Mediated Synthesis of Silver Nanoparticles

Abstract

Green synthesis of nanoparticles (NPs) using bacteria offers advantages of being eco-friendly, cost-effective and nontoxic. In the present study, cell cell-free culture supernatant of Chitinophaga sp. S167 was utilized for the bio-fabrication of silver nanoparticles (AgNPs). The physicochemical properties of AgNPs were studied using UV–visible spectroscopy, transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopic analysis (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). The biogenic synthesized AgNPs showed higher growth inhibitory efficacy against the tested Gram-positive and Gram-negative bacteria, fungi and clinical isolates of Candida glabrata as compared to the chemically synthesized AgNPs using sodium borohydride. Further, the biogenic AgNPs showed potent antiproliferative activity against the two human cancer cell lines (HCT-116 [colon cancer] and MG-63 [osteosarcoma]) as compared to the chemically synthesized AgNPs. This is the first study reporting the biogenic synthesis of AgNPs using Chitinophaga sp. S167, demonstrating their potent antibacterial, antifungal and anticancer activities.