The critical role of transcription factor GATA1 in erythrocyte proliferation and morphology in blood clam Anadara granosa

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-27 DOI:10.1016/j.ijbiomac.2025.144691

Jiacheng Wang , Yuhang Zhang , Yingbin Xu , Hongyu Jin , Can Yang , Hongxing Liu , Yongbo Bao

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引用次数: 0

Abstract

The blood clam (Anadara granosa) exhibits innovative red blood compared to other molluscs, with erythrocytes constituting 79 % to 89 % of the total hemocyte count. Previously, we identified that GATA-binding protein 1 (designated as AgGATA1) was significantly up-regulated in the transcriptomes of blood clam populations with high hemocyte counts. However, the function of AgGATA1 in erythropoiesis remains unknown. In this study, we conducted GATA1 knockdown experiments in A. granosa, which resulted in a significant reduction of both erythrocytes and other cells during the S phase, as well as significantly decreased proliferative activity in the gill tissue. The cell proliferation assay demonstrated that overexpression of AgGATA1 increased the DNA replication activity of K562 cells. Additionally, o-dianisidine staining revealed defects in erythropoiesis within the gill tissue following AgGATA1 knockdown, while protein analyses confirmed a significant reduction in AgGATA1 expression. Furthermore, decreased cytoskeletal fluorescence and morphological changes in erythrocytes suggest that AgGATA1 influenced cytoskeletal protein expression, leading to changes in erythrocyte morphology. This study provides new insights into the role of AgGATA1 in hemocyte formation and highlights its significance in understanding erythropoiesis within invertebrate developmental tissues.

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转录因子GATA1在血蛤红细胞增殖和形态中的关键作用

与其他软体动物相比，血蛤（Anadara granosa）具有创新的红色血液，其红细胞占血细胞总数的79%至89%。先前，我们发现gata结合蛋白1（被称为AgGATA1）在血细胞计数高的血蛤种群的转录组中显著上调。然而，AgGATA1在红细胞生成中的功能尚不清楚。在本研究中，我们对a . granosa进行了GATA1敲低实验，导致S期红细胞和其他细胞显著减少，同时鳃组织的增殖活性显著降低。细胞增殖实验表明，过表达AgGATA1增加了K562细胞的DNA复制活性。此外，o-dianisidine染色显示AgGATA1敲低后，腮组织内红细胞生成缺陷，而蛋白质分析证实AgGATA1表达显著降低。此外，红细胞的细胞骨架荧光减少和形态学改变表明，AgGATA1影响了细胞骨架蛋白的表达，导致红细胞形态学的改变。本研究为AgGATA1在血细胞形成中的作用提供了新的见解，并强调了其在理解无脊椎动物发育组织中红细胞生成的意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.