Structural characterization and anticancer properties of a novel mushroom-derived rhamnogalactan in a triple-negative breast cancer model

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-05-23 DOI:10.1016/j.carbpol.2025.123735

Eliane Leal de Lara , Matheus Zavadinack , Shayane da Silva Milhorini , Karin Maciel Wurzer , Giseli Klassen , Lucimara M.C. Cordeiro , Marcello Iacomini

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引用次数: 0

Abstract

Mushroom-derived polysaccharides are known for their diverse biological activities, including immunomodulatory and anticancer properties. Breast cancer, the leading cause of cancer-related death among women worldwide, lacks targeted molecular therapies, underscoring the need for novel therapeutic agents. In this study, we extracted, purified, and characterized a novel heteropolysaccharide (HTP) - a rhamnogalactan - composed of galactose (72.6 %) and rhamnose (27.4 %) from the edible mushroom Lactarius quieticolor. Structural analysis revealed that HTP consists of a (1 → 6)-linked α-d-galactopyranose backbone, heavily substituted at O-2 by non-reducing rhamnose units, with a weight-average molar mass of 2.41 × 10⁴ g.mol⁻¹. In vitro assays using a triple-negative breast cancer (TNBC) cell model - the most aggressive subtype - showed a significant reduction in cell viability (50 % at 1200 μg·mL⁻¹). Furthermore, an additive effect was observed when co-administered with the chemotherapy drug paclitaxel, reducing the cell viability to 42 %. These findings suggest potential therapeutic applications of the purified mushroom rhamnogalactan reported in this study against TNBC.

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一种新型蘑菇衍生鼠李糖半乳聚糖在三阴性乳腺癌模型中的结构表征和抗癌特性

蘑菇衍生的多糖以其多种生物活性而闻名，包括免疫调节和抗癌特性。乳腺癌是全世界妇女癌症相关死亡的主要原因，缺乏靶向分子疗法，这突出了对新型治疗剂的需求。本研究从食食菌Lactarius quieticolor中提取、纯化并鉴定了一种新型半乳糖(HTP) -鼠李糖半乳聚糖（rhamno半乳聚糖），该多糖由半乳糖（72.6%）和鼠李糖（27.4%）组成。结构分析表明，HTP由（1→6）键连接的α-d-半乳糖糖骨架组成，在O-2位置被不还原鼠李糖单元大量取代，其重量平均摩尔质量为2.41 × 104 g.mol−1。使用三阴性乳腺癌（TNBC）细胞模型（最具侵袭性的亚型）进行的体外实验显示，在1200 μg·mL−1时，细胞活力显著降低（50%）。此外，当与化疗药物紫杉醇共同施用时，观察到一种附加效应，将细胞活力降低到42%。这些发现提示了本研究中报道的纯化蘑菇鼠李糖半乳聚糖对TNBC的潜在治疗应用。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.