Dual nanozyme bubble-propelled biosensor for multiple miRNAs detection overcomes spatial hurdles of DNA probes

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-23 DOI:10.1016/j.bios.2025.117624

Xiaofang Liu , Guangli Luo , Fan Shi , Qun Wang , Zhan Diao , Liang Dong , Fei Deng , Danqun Huo , Changjun Hou

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引用次数: 0

Abstract

Liquid biopsy offers a promising approach for cancer detection, with biosensors enhancing its practicality and applicability. In this study, we propose a biosensor that utilizes the synergistic catalytic release of fuel-generated bubbles by AuCuMn and CaH to improve detection efficiency. The bubble propulsion of DNA probes accelerates receptor-target interactions, significantly enhancing the biosensor's performance. Specifically, when detecting miRNA-21, bubble propulsion lowered the minimum detectable concentration from 3.2 pmol/L to 640 fmol/L, achieving a fivefold increase in sensitivity. Furthermore, the DNA probe in the biosensor can be modularly replaced to detect other miRNAs, such as miRNA-96, miRNA-155, and miRNA-210. These miRNAs can be quantitatively detected within the range of 640 fmol/L to 50 nmol/L, exhibiting excellent specificity, reproducibility and stability. Abnormal expression of these miRNAs was clearly observed in HER2+ breast cancer patients' serum. These findings not only confirm the biosensor's effectiveness in detecting cancer-related miRNAs but also provide valuable insights for its future clinical translation and application.

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双纳米酶气泡推进型多mirna生物传感器克服了DNA探针的空间障碍

液体活检为癌症检测提供了一种很有前途的方法，生物传感器增强了它的实用性和适用性。在本研究中，我们提出了一种利用AuCuMn和CaH协同催化释放燃料产生的气泡来提高检测效率的生物传感器。DNA探针的气泡推进加速了受体与靶标的相互作用，显著提高了生物传感器的性能。具体来说，当检测miRNA-21时，气泡推进将最小可检测浓度从3.2 pmol/L降低到640 fmol/L，实现了灵敏度的五倍提高。此外，生物传感器中的DNA探针可以模块化地替换，以检测其他mirna，如miRNA-96， miRNA-155和miRNA-210。这些mirna可在640 fmol/L ~ 50 nmol/L范围内定量检测，具有良好的特异性、重复性和稳定性。这些mirna在HER2阳性乳腺癌患者血清中明显表达异常。这些发现不仅证实了该生物传感器在检测癌症相关mirna方面的有效性，也为其未来的临床转化和应用提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.