AgAu@Ag core-shell triangular nanonet jointed with composite SERS enhanced substrate for capturing and sensing urine biomarkers FGFR3 and NMP22

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-26 DOI:10.1016/j.bios.2025.117635

Hang-Zhuo Li , Jian Zhu , Guo-Jun Weng , Jian-Jun Li , Lei Li , Jun-Wu Zhao

{"title":"AgAu@Ag core-shell triangular nanonet jointed with composite SERS enhanced substrate for capturing and sensing urine biomarkers FGFR3 and NMP22","authors":"Hang-Zhuo Li , Jian Zhu , Guo-Jun Weng , Jian-Jun Li , Lei Li , Jun-Wu Zhao","doi":"10.1016/j.bios.2025.117635","DOIUrl":null,"url":null,"abstract":"<div><div>Herein, we achieved ultrasensitive surface-enhanced Raman scattering (SERS) detection of bladder cancer (BC) urine biomarkers, nuclear matrix protein 22 (NMP22) and fibroblast growth factor receptor 3 (FGFR3) by preparing gold and silver core-shell triangular nanonets (AgAu@Ag TNNs) with porous triangular mesh structures. The tip effect of the triangular structure of AgAu@Ag TNNs and the plasma coupling of the mesh holes on its surface enabled an enhancement factor (EF) of 1.37 × 10<sup>8</sup>. Moreover, we prepared gold-sprayed silicon wafers (Si@Au) by a simple physical method and covered graphene oxide (GO) on them as SERS substrates (Si@Au@GO). The plasmon coupling effect and chemical enhancement of this substrate improved the EF of AgAu@Ag TNNs to 1.88 × 10<sup>8</sup>. Based on above excellent SERS performance of nanoparticles combined with substrate, we achieved simultaneous quantitative detection of FGFR3 and NMP22 in the range of 100 fg/mL- 10 ng/mL and 100 fg/mL- 100 ng/mL, with the limit of detection (LOD) of 73.36 fg/mL and 21.56 fg/mL, respectively. This ultra-sensitive and specific joint immunosensor provides a reliable detection strategy to assist in the precise localisation of BC clinical staging.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"286 ","pages":"Article 117635"},"PeriodicalIF":10.7000,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325005093","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Herein, we achieved ultrasensitive surface-enhanced Raman scattering (SERS) detection of bladder cancer (BC) urine biomarkers, nuclear matrix protein 22 (NMP22) and fibroblast growth factor receptor 3 (FGFR3) by preparing gold and silver core-shell triangular nanonets (AgAu@Ag TNNs) with porous triangular mesh structures. The tip effect of the triangular structure of AgAu@Ag TNNs and the plasma coupling of the mesh holes on its surface enabled an enhancement factor (EF) of 1.37 × 10⁸. Moreover, we prepared gold-sprayed silicon wafers (Si@Au) by a simple physical method and covered graphene oxide (GO) on them as SERS substrates (Si@Au@GO). The plasmon coupling effect and chemical enhancement of this substrate improved the EF of AgAu@Ag TNNs to 1.88 × 10⁸. Based on above excellent SERS performance of nanoparticles combined with substrate, we achieved simultaneous quantitative detection of FGFR3 and NMP22 in the range of 100 fg/mL- 10 ng/mL and 100 fg/mL- 100 ng/mL, with the limit of detection (LOD) of 73.36 fg/mL and 21.56 fg/mL, respectively. This ultra-sensitive and specific joint immunosensor provides a reliable detection strategy to assist in the precise localisation of BC clinical staging.

查看原文本刊更多论文

AgAu@Ag核壳三角形纳米网络与复合SERS增强底物连接，用于捕获和检测尿液生物标志物FGFR3和NMP22

在此，我们通过制备具有多孔三角形网状结构的金和银核壳三角形纳米网（AgAu@Ag TNNs），实现了对膀胱癌（BC）尿液生物标志物、核基质蛋白22 （NMP22）和成纤维细胞生长因子受体3 （FGFR3）的超灵敏表面增强拉曼散射（SERS）检测。AgAu@Ag TNNs三角形结构的尖端效应和表面网眼的等离子体耦合使其增强因子（EF）达到1.37 × 108。此外，我们通过简单的物理方法制备了镀金硅片（Si@Au），并在其上覆盖氧化石墨烯（GO）作为SERS衬底（Si@Au@GO）。该衬底的等离子体耦合效应和化学增强使AgAu@Ag TNNs的等效系数提高到1.88 × 108。基于上述纳米颗粒与底物结合的优越SERS性能，我们实现了FGFR3和NMP22在100 fg/mL- 10 ng/mL和100 fg/mL- 100 ng/mL范围内的同时定量检测，检出限（LOD）分别为73.36 fg/mL和21.56 fg/mL。这种超灵敏和特异性的关节免疫传感器提供了一种可靠的检测策略，以协助精确定位BC临床分期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.