Mechanically tuned dual-network scaffolds orchestrate neuro-angio-osteo coupling for enhanced bone regeneration

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-05-27 DOI:10.1016/j.cej.2025.164176

Mengmeng Yang, Yang Liu, Wenjie Li, Pengyin Li, Shaoqing Chen, Chun Liu, Bang An, Qiangsheng Dong, Cheng Wang, Feng Xue, Chenglin Chu, Jing Bai, Qiangrong Gu, Xinye Ni

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Abstract

Current bone grafts often lack the complex regenerative cues required for complete healing. This study introduced a dual-network scaffold composed of amorphous magnesium calcium pyrophosphate (AMCP), cassava starch (CS), and polyvinyl alcohol (PVA), engineered to overcome these limitations. PVA integration reinforced hydrogen bonding within the scaffold, enhancing mechanical properties and orchestrating a controlled three-stage degradation process. This included mitigating initial swelling, promoting cell adhesion via improved surface roughness, and stabilizing the structure during bone ingrowth. The resulting construct promoted neurovascular bone repair by enhancing cell adhesion/proliferation while retaining ion release capacity. Mechanistically, the scaffold activated the PI3K-AKT pathway in osteoblasts (ROCK upregulation, ATP boost via Ppa1/ATP synthase), stimulated angiogenesis (VEGFA, Serpine1, VEGFR1, Cdkn1a), and promoted neurogenesis (Wasf1, Limk2). VEGFA-mediated endothelial cell activation appeared central to coordinating the process, leading to enhanced neuro-angio-osteo interactions. Thus, the AMCP/CS/PVA scaffold provided a promising biomimetic platform for spatiotemporally coordinated bone regeneration by integrating multifaceted mechanical cues and regenerative signals.

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机械调谐双网络支架协调神经-血管-骨耦合以增强骨再生

目前的骨移植通常缺乏完全愈合所需的复杂的再生线索。本研究介绍了一种由无定形焦磷酸钙镁（AMCP）、木薯淀粉（CS）和聚乙烯醇（PVA）组成的双网络支架，旨在克服这些局限性。聚乙烯醇集成增强了支架内的氢键，提高了机械性能，并协调了一个可控的三阶段降解过程。这包括减轻初始肿胀，通过改善表面粗糙度促进细胞粘附，以及在骨长入过程中稳定结构。由此产生的结构通过增强细胞粘附/增殖同时保持离子释放能力来促进神经血管骨修复。在机制上，支架激活了成骨细胞中的PI3K-AKT通路（ROCK上调，通过Ppa1/ATP合成酶增加ATP），刺激血管生成（VEGFA, Serpine1, VEGFR1, Cdkn1a），促进神经发生（Wasf1, Limk2）。vegf介导的内皮细胞活化似乎是协调这一过程的核心，导致神经-血管-骨相互作用增强。因此，AMCP/CS/PVA支架通过整合多方面的机械线索和再生信号，为骨的时空协调再生提供了一个很有前景的仿生平台。

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.