Detection of DENV in Aedes albopictus during the 2024 sporadic dengue outbreak in Wuhan city, Hubei Province, China.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-05-24 DOI:10.1186/s12985-025-02763-1

Liqun Wu, Yijie Zhang, Zhi Chen, Banghua Chen, Manqing Liu, Weifeng Tang, Feng Pan, Wenhua Kong, Yixuan Wu, Xiaomin Chen

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引用次数: 0

Abstract

From September to October 2024 in Wuhan, three indigenous dengue cases were reported in Wuhan for the first time since the 1940s. Here, we report the results of the epidemiological investigation of the sporadic dengue outbreak and the detection of dengue virus (DENV) in Aedes (Stegomyia) albopictus (Skuse) (Diptera: Culicidae) collected from locations surrounding the living and working places of patients. A total of 190 female and 56 male Ae. albopictus mosquitoes were collected using a combination of BG-Mosquitaire trap and human-baited double net (HDN) trap during the outbreak response. DENV RNA was detected via RT-PCR in patient serum and mosquito samples. Among the Aedes mosquitoes tested, DENV-1 RNA was detected in two samples, with an average MIR of 10.5, but only one of which achieved the DENV sequence. Phylogenetic analysis showed that the virus strain detected in the mosquito sample, sharing 100% homology with that in the patient serum sample, belonged to genotype 1IK.2 and was closely related to strains circulating in Guangzhou in 2023. This is the first report of the detection of DENV in Ae. albopictus during a sporadic autochthonous dengue sporadic outbreak in Wuhan.

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2024年湖北省武汉市登革热散发疫情期间白纹伊蚊DENV的检测

2024年9 - 10月，武汉市自20世纪40年代以来首次报告3例本土登革热病例。本文报道了本地区散发型登革热暴发的流行病学调查结果和在患者生活和工作场所周围采集白纹伊蚊（双翅目：库蚊科）的登革热病毒（DENV）检测结果。其中雌伊蚊190只，雄伊蚊56只。在疫情应对期间，采用bg -诱蚊器和人饵双网（HDN）诱蚊器联合采集白纹伊蚊。采用RT-PCR检测患者血清和蚊虫样本中DENV RNA。在2份样本中检测到DENV-1 RNA，平均MIR为10.5，但只有1份样本获得了DENV序列。系统发育分析表明，蚊虫样本中检测到的病毒株与患者血清样本同源性为100%，为基因型1IK。2株与2023年广州流行株密切相关。这是首例登革热病毒在伊蚊中检测到的报告。在武汉市发生的登革热散发疫情中发现白纹伊蚊。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.