Rhamnolipids production in semi-submerged static-cultivation using agar cubes as solid substrate.

IF 4.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-05-26 DOI:10.1007/s11274-025-04412-9

Mayara de Alencar Almeida, Karen Stefany Conceição, Juliana Perez Marques Teixeira, Nicole Caldas Pan, Luis Paulo Silveira Alves, Gerson Nakazato, Lucélia Donatti, Emanuel M Souza, Doumit Camilios-Neto, Josiane A Vignoli

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引用次数: 0

Abstract

Surfactants are amphiphilic molecules with a worldwide billionaire market. Although chemical surfactants are the most available and commonly used, the microbiologically produced congeners have attracted attention due to their low toxicity, biodegradability, and high ecological acceptability. Among microbial surfactants rhamnolipids stand out, with high yields of production, excellent surface-active properties and great potential market. However, the application of these compounds is still limited mostly because of excessive production costs. This study presents a cost-effective process for rhamnolipids production by semi-submerged static-cultivation using agar cubes as substrate. A concentration of 50 g/L of rhamnolipids was reached in semi-submerged static-cultivation using agar cubes containing corn bran and soybean oil. Through this static process, besides avoiding foam, gradual release of nutrients provides a large increase in P. aeruginosa growth and consequently on rhamnolipids production.

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以琼脂块为固体基质的半淹没静态培养中鼠李糖脂的生产。

表面活性剂是一种两亲性分子，在全球有着巨大的市场。虽然化学表面活性剂是最容易获得和最常用的，但微生物生产的同系物因其低毒性、生物降解性和高生态可接受性而受到人们的关注。在微生物表面活性剂中，鼠李糖脂以其高产率、优异的表面活性性能和巨大的市场潜力而引人注目。然而，由于生产成本过高，这些化合物的应用仍然受到限制。本研究提出了一种以琼脂块为底物的半淹没静态培养生产鼠李糖脂的低成本工艺。用含有玉米皮和大豆油的琼脂块进行半淹没静态培养，鼠李糖脂的浓度达到50 g/L。通过这种静态过程，除了避免泡沫外，营养物质的逐渐释放大大增加了铜绿假单胞菌的生长，从而增加了鼠李糖脂的产生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.