{"title":"Pilot Study: Lymphatic Brain Decongestion: A New Surgical Technique in Alzheimer Disease.","authors":"Anastasios Topalis, Chrysanthi Bekiari, Efterpi Demiri, Dimitrios Dionyssiou","doi":"10.1097/GOX.0000000000006806","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and affect neurodegeneration by transferring amyloid-beta peptides from the brain to the cervical lymph nodes. We aimed to assess the feasibility of a novel operation in a rat model, to enhance the meningeal lymphatic system and consequently increase the clearance of amyloid-beta peptide.</p><p><strong>Methods: </strong>Ten 12-month-old rats were divided into 2 groups. Group A (control) had no intervention. Group B underwent collagen scaffold implantation between the subdural space above the hippocampus and the right submandibular lymph node, to achieve guided lymphangiogenesis. Animals were weighed and examined for neurological, behavioral and cognitive tests preoperatively and 6 and 12 weeks postoperatively, including NeuroScale, modified open field, and novel object location recognition tests.</p><p><strong>Results: </strong>One animal of group B died 2 days postoperatively. The remaining rats had no complications, maintained or increased their weight, and showed no signs of infection or neurological impairment. Behavioral tests were in favor of group B at 12 weeks postoperative. Animals of group A had statistically significant decline in cognitive function over time. Animals of group B demonstrated the same or better cognitive function.</p><p><strong>Conclusions: </strong>In this pilot study, we demonstrated the feasibility of the proposed surgical procedure, which resulted in positive cognitive function for the animals. We are now utilizing an AD rat model to evaluate the possible therapeutic effect of the procedure.</p>","PeriodicalId":20149,"journal":{"name":"Plastic and Reconstructive Surgery Global Open","volume":"13 5","pages":"e6806"},"PeriodicalIF":1.5000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101920/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plastic and Reconstructive Surgery Global Open","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/GOX.0000000000006806","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Alzheimer disease (AD) is characterized by progressive cognitive function decline and neurodegeneration. Accumulation of amyloid-beta peptides forms plaques leading to brain inflammation and neuronal death. Glymphatic and meningeal lymphatic systems regulate brain fluid homeostasis and affect neurodegeneration by transferring amyloid-beta peptides from the brain to the cervical lymph nodes. We aimed to assess the feasibility of a novel operation in a rat model, to enhance the meningeal lymphatic system and consequently increase the clearance of amyloid-beta peptide.
Methods: Ten 12-month-old rats were divided into 2 groups. Group A (control) had no intervention. Group B underwent collagen scaffold implantation between the subdural space above the hippocampus and the right submandibular lymph node, to achieve guided lymphangiogenesis. Animals were weighed and examined for neurological, behavioral and cognitive tests preoperatively and 6 and 12 weeks postoperatively, including NeuroScale, modified open field, and novel object location recognition tests.
Results: One animal of group B died 2 days postoperatively. The remaining rats had no complications, maintained or increased their weight, and showed no signs of infection or neurological impairment. Behavioral tests were in favor of group B at 12 weeks postoperative. Animals of group A had statistically significant decline in cognitive function over time. Animals of group B demonstrated the same or better cognitive function.
Conclusions: In this pilot study, we demonstrated the feasibility of the proposed surgical procedure, which resulted in positive cognitive function for the animals. We are now utilizing an AD rat model to evaluate the possible therapeutic effect of the procedure.
期刊介绍：
Plastic and Reconstructive Surgery—Global Open is an open access, peer reviewed, international journal focusing on global plastic and reconstructive surgery.Plastic and Reconstructive Surgery—Global Open publishes on all areas of plastic and reconstructive surgery, including basic science/experimental studies pertinent to the field and also clinical articles on such topics as: breast reconstruction, head and neck surgery, pediatric and craniofacial surgery, hand and microsurgery, wound healing, and cosmetic and aesthetic surgery. Clinical studies, experimental articles, ideas and innovations, and techniques and case reports are all welcome article types. Manuscript submission is open to all surgeons, researchers, and other health care providers world-wide who wish to communicate their research results on topics related to plastic and reconstructive surgery. Furthermore, Plastic and Reconstructive Surgery—Global Open, a complimentary journal to Plastic and Reconstructive Surgery, provides an open access venue for the publication of those research studies sponsored by private and public funding agencies that require open access publication of study results. Its mission is to disseminate high quality, peer reviewed research in plastic and reconstructive surgery to the widest possible global audience, through an open access platform. As an open access journal, Plastic and Reconstructive Surgery—Global Open offers its content for free to any viewer. Authors of articles retain their copyright to the materials published. Additionally, Plastic and Reconstructive Surgery—Global Open provides rapid review and publication of accepted papers.
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