Characterization studies on the 05SSU0969 gene of Streptococcus suis serotype 2 in adhesion, anti-phagocytosis, and virulence in animal.

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-08-01 Epub Date: 2025-05-23 DOI:10.1016/j.micpath.2025.107739

Yaya Pian, Yaxuan Wang

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引用次数: 0

Abstract

Objective: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, however, the pathogenesis of SS2 remains unclear. Our objective was to study the role of the type IV secretion system (T4SS) 05SSU0969 gene in SS2 virulence.

Methods: To assess the function of 05SSU0969 in SS2 virulence, the wild-type strain 05ZYH33 served as the parent strain. Using homologous recombination technology, the mutant strain Δ0969 and the complemented strain CΔ0969 were generated. The biological properties of these strains were evaluated through growth curve analysis, cultivation characteristics, cell adhesion assays, blood survival assays, and pathogenicity tests in CD1 mice and piglets.

Results: Growth curve analysis revealed no significant differences among 05ZYH33, Δ0969, and CΔ0969. Although Δ0969 exhibited similar cultivation characteristics to 05ZYH33 on sheep blood agar, its adhesion to A549, Hep-2, and HBMEC cells was significantly impaired. Additionally, Δ0969 demonstrated markedly reduced blood survival and attenuated virulence in both mice and piglets models.

Conclusions: These findings indicate that deletion of 05SSU0969 gene attenuates SS2 resistance and virulence in CD1 mice and piglets. However, the precise mechanism by which 05SSU0969 gene influences adhesion to A549, Hep-2, and HBMEC cells requires further investigation.

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猪链球菌血清2型05SSU0969基因对动物黏附、抗吞噬和毒力的特性研究。

目的：猪链球菌血清2型（SS2）是一种重要的人畜共患病原体，但其发病机制尚不清楚。我们的目的是研究IV型分泌系统（T4SS） 05SSU0969基因在SS2毒力中的作用。方法：以野生型菌株05ZYH33为亲本菌株，评价05SSU0969对SS2毒力的作用。利用同源重组技术，得到突变株Δ0969和互补株CΔ0969。通过生长曲线分析、培养特性、细胞粘附试验、血液存活试验和CD1小鼠和仔猪致病性试验来评价这些菌株的生物学特性。结果：生长曲线分析显示05ZYH33与Δ0969、CΔ0969之间无显著差异。虽然Δ0969在羊血琼脂上表现出与05ZYH33相似的培养特性，但其对A549、Hep-2和HBMEC细胞的粘附性明显受损。此外，Δ0969在小鼠和仔猪模型中均表现出明显降低血液存活率和减弱毒力。结论：上述结果表明，05SSU0969基因的缺失可降低CD1小鼠和仔猪对SS2的耐药性和毒力。然而，05SSU0969基因影响A549、Hep-2和HBMEC细胞粘附的确切机制需要进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)