Tímea Bálint, Mihály Ruppert, Bence Ágg, Dávid Nagy, Krisztina Pálóczi, Kálmán Szenthe, Ferenc Bánáti, Alex Ali Sayour, Attila Oláh, Bálint András Barta, Javier Barallobre-Barreiro, Péter Ferdinandy, Béla Merkely, Tamás Radovits
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引用次数: 0
Abstract
Background: Atrial fibrillation (AF) is common in patients with chronic heart failure (HF). Nevertheless, some patients with HF remain in sinus rhythm (SR) even with marked left atrial (LA) dilatation and fibrosis. The underlying mechanisms for the differences in atrial arrhythmogenicity are poorly uncovered. Recent findings indicate that distinct microRNAs (miRNA) might induce LA structural and molecular alterations. However, the impact of miRNA dysregulation on AF development in the context of HF has not been studied independently of LA remodeling.
Objective: This study aimed to evaluate the differences in LA miRNA expressions in HF patients with AF or SR.
Methods: LA myocardial samples were obtained from advanced HF patients with AF (n=12; paroxysmal n=4, chronic as persistent/permanent n=8) or SR (n=12) undergoing heart transplantation. The extent of LA interstitial fibrosis was evaluated using picrosirius red-staining. The LA load was estimated by measuring LA mRNA expression of the NPPA gene encoding atrial natriuretic peptide with qRT-PCR and circulating N-terminal proatrial natriuretic peptide (NT-proANP) by ELISA. The LA miRNA screening was performed using the NanoString technology.
Results: LA dilatation, fibrosis, NPPA gene expression, as well as circulating NT-proANP levels were similar between the AF and SR groups, suggesting a comparable extent of atrial remodeling and load among the study groups. The miRNA analysis revealed no differences in atrial miRNA expression between the groups, even after AF subgroup analysis.
Conclusion: The LA miRNA expression profile shows no distinction between AF and SR in advanced HF patients with similar levels of pathological atrial remodeling.
期刊介绍：
HeartRhythm, the official Journal of the Heart Rhythm Society and the Cardiac Electrophysiology Society, is a unique journal for fundamental discovery and clinical applicability.
HeartRhythm integrates the entire cardiac electrophysiology (EP) community from basic and clinical academic researchers, private practitioners, engineers, allied professionals, industry, and trainees, all of whom are vital and interdependent members of our EP community.
The Heart Rhythm Society is the international leader in science, education, and advocacy for cardiac arrhythmia professionals and patients, and the primary information resource on heart rhythm disorders. Its mission is to improve the care of patients by promoting research, education, and optimal health care policies and standards.
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