Identification and validation of USP15 and CUL2 as ubiquitination related biomarker in chronic obstructive pulmonary disease.

IF 2.7 3区生物学

Hereditas Pub Date : 2025-05-24 DOI:10.1186/s41065-025-00460-1

Shulei Sun, Zhaoxiong Zhang, Haiyan Zhao

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引用次数: 0

Abstract

Purpose: Ubiquitination is one of the important epigenetic modifications, influencing the development of various diseases. The objective of this study is to investigate the ubiquitination related genes in chronic obstructive pulmonary disease (COPD).

Methods: The gene microarray dataset from COPD patients and ubiquitination related genes were analyzed. Venn diagram analysis was used to intersect differentially expressed genes and ubiquitination related genes. The functional enrichment analysis of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) were performed on differentially expressed ubiquitination related genes. Finally, we confirmed the expression of hub genes through qPCR and western blot experiments in clinical COPD patients and cell lines.

Results: We identified 2,932 differentially expressed genes and 96 differentially expressed ubiquitination related genes. GO analysis indicated that the differentially expressed ubiquitination related genes were mainly enriched in post-translational protein modification and ubiquitin ligase complex. KEGG analysis showed that ubiquitination related genes were mainly involved in ubiquitin mediated proteolysis and TNF signaling pathway. GSEA analysis suggested that some hub genes are involved in allograft rejection, IL6/JAK/STAT3 signaling and inflammatory response. Our qPCR and western blot experimental results indicate that the expression of USP15 and CUL2 is higher in COPD group compared to the control group, consistent with the bioinformatics analysis.

Conclusion: Our bioinformatics analysis and experimental results suggest that USP15 and CUL2 may contribute to the progression of COPD through ubiquitination modification. To our knowledge, this is the first study to demonstrate the involvement of USP15 and CUL2 in COPD. Our results may provide new insights into the diagnosis and treatment of COPD.

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USP15和CUL2作为慢性阻塞性肺疾病泛素化相关生物标志物的鉴定和验证

目的：泛素化是影响多种疾病发生发展的重要表观遗传修饰之一。本研究的目的是探讨慢性阻塞性肺疾病（COPD）中泛素化相关基因。方法：分析COPD患者基因芯片数据集及泛素化相关基因。用维恩图分析交叉差异表达基因和泛素化相关基因。通过基因本体（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）对差异表达的泛素化相关基因进行功能富集分析。最后，我们通过qPCR和western blot实验在临床COPD患者和细胞系中证实hub基因的表达。结果：共鉴定出2932个差异表达基因和96个差异表达泛素化相关基因。GO分析表明，差异表达的泛素化相关基因主要富集于翻译后蛋白修饰和泛素连接酶复合体。KEGG分析显示泛素化相关基因主要参与泛素介导的蛋白水解和TNF信号通路。GSEA分析表明，一些中枢基因参与了同种异体移植排斥反应、IL6/JAK/STAT3信号传导和炎症反应。我们的qPCR和western blot实验结果显示，COPD组中USP15和CUL2的表达高于对照组，与生物信息学分析一致。结论：我们的生物信息学分析和实验结果提示USP15和CUL2可能通过泛素化修饰参与COPD的进展。据我们所知，这是第一个证明USP15和CUL2参与COPD的研究。我们的结果可能为COPD的诊断和治疗提供新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.