Identification of RMP24 and RMP64, human ribonuclease MRP-specific protein components.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-05-24 DOI:10.1016/j.celrep.2025.115752

Rui Che, Bhoomi Mirani, Monireh Panah, Xiaotong Chen, Hong Luo, Andrei Alexandrov

引用次数: 0

Abstract

Human RNase MRP is a ribonucleoprotein (RNP) enzyme that processes precursor rRNA (pre-rRNA) at ITS1 site 2 and may have additional activities. It is an endonuclease related to RNase P, which processes pre-tRNAs and pre-tRNA-like substrates. In Saccharomyces cerevisiae, these two RNPs utilize distinct catalytic RNAs with eight shared and one or two specific protein subunits. However, the human RNase MRP-specific protein subunits remain unidentified. Our genome-wide forward genetic screening identifies two poorly characterized human genes, which we name ribonuclease MRP subunit P24 (RMP24) and RMP64. We show that Rmp24 and Rmp64 are required for pre-rRNA ITS1 site 2 processing and associate with MRP RNA but are not required for RNase P activity and do not associate with RNase P-specific H1 RNA. Despite limited sequence homology, Rmp24 and Rmp64 exhibit predicted structural similarities to two RNase MRP-specific components in S. cerevisiae. Collectively, our functional screening and validation reveal two protein components unique to human nuclear RNase MRP.

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鉴定人核糖核酸酶mrp特异性蛋白组分RMP24和RMP64。

人类RNase MRP是一种核糖核蛋白（RNP）酶，在ITS1位点2加工前体rRNA (pre-rRNA)，并可能具有其他活性。它是一种与RNase P相关的内切酶，处理前trna和前trna样底物。在酿酒酵母中，这两种RNPs利用不同的催化rna，具有8个共享的和一个或两个特定的蛋白质亚基。然而，人类RNase mrp特异性蛋白亚基仍未确定。我们的全基因组正向遗传筛选鉴定了两个特征较差的人类基因，我们将其命名为核糖核酸酶MRP亚基P24 （RMP24）和RMP64。我们发现Rmp24和Rmp64是pre-rRNA ITS1 site 2加工所必需的，并与MRP RNA相关联，但不是RNase P活性所必需的，也不与RNase P特异性H1 RNA相关联。尽管序列同源性有限，但Rmp24和Rmp64表现出与酿酒酵母中两个RNase mrp特异性成分的预测结构相似性。总的来说，我们的功能筛选和验证揭示了人类核rna酶MRP特有的两种蛋白质成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

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