Early-life development of the gut virome and plasmidome: A longitudinal study in cesarean-born infants.

IF 6.9 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-06-24 Epub Date: 2025-05-23 DOI:10.1016/j.celrep.2025.115731

Asier Fernández-Pato, Trishla Sinha, Sanzhima Garmaeva, Anastasia Gulyaeva, Nataliia Kuzub, Simon Roux, Jingyuan Fu, Alexander Kurilshikov, Alexandra Zhernakova

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引用次数: 0

Abstract

Mobile genetic elements (MGE) are critical yet understudied determinants of gut microbiome composition. In this secondary analysis of a randomized controlled trial (NCT06030713), we characterized the gut virome and plasmidome in 195 samples from 28 mother-infant dyads delivered by cesarean section. Infant mobilome increases in richness over the first 6 postnatal weeks, demonstrating high individual-specificity and temporal stability, establishing a personal persistent mobilome. Formula-fed infants exhibit greater mobilome richness than breastfed infants, with plasmid composition being influenced by antibiotic exposure and birth weight. Plasmids constitute a reservoir of antibiotic resistance genes (ARG), with around 5% of infant gut plasmid taxonomic units carrying ARG. Notably, ARG profiles do not differ with antibiotic exposure at birth. Mother-infant sharing of viral and plasmid strains primarily occurs after 6 months of age. Overall, our integrative analysis offers insights into the dynamics, modulation, and origin of MGE in the developing gut microbiome.

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肠道病毒和质粒的早期发育：对剖宫产婴儿的纵向研究。

移动遗传因子（MGE）是肠道微生物组组成的关键因素，但尚未得到充分研究。在这项随机对照试验（NCT06030713）的二级分析中，我们对剖宫产的28对母婴的195个样本的肠道病毒组和质粒进行了表征。婴儿移动组的丰富度在出生后的前6周增加，表现出高度的个体特异性和时间稳定性，建立了个人持久的移动组。配方奶喂养的婴儿比母乳喂养的婴儿表现出更大的移动组丰富度，质粒组成受到抗生素暴露和出生体重的影响。质粒是抗生素耐药基因（ARG）的储存库，约5%的婴儿肠道质粒分类单位携带ARG。值得注意的是，ARG谱与出生时的抗生素暴露没有差异。母婴共享病毒和质粒株主要发生在6个月后。总的来说，我们的综合分析提供了对发育中的肠道微生物组中MGE的动力学，调节和起源的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.