The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1.

Abstract

Background: To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN).

Methods: After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR).

Results: Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (P < 0.05). The optimal coculture model with Acinetobacter lwoffii was an MOI of 100 following 18 h-24 h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12 h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12 h to 24 h (all P < 0.05).

Conclusion: Acinetobacter lwoffii can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner.