Biochemical and histopathological changes in ovary, uterus and testicular tissues after acrylamide exposure

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-05-26 DOI:10.1007/s10735-025-10456-w

Hamit Uslu, Gözde Atila Uslu, Taha Abdulkadir Çoban, Ali Sefa Mendil, Emine Toraman, Mahmut Şahin, Mustafa Özkaraca

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引用次数: 0

Abstract

Acrylamide (ACR) is a popular substance to which our exposure increases with the changes in our lifestyle and brings with it various health problems. In order to determine appropriate therapeutics against ACR damage, it is important to investigate the multiple mechanisms that may be effective in its pathophysiology. This study investigated the effects of ACR exposure on ovarian, uterine, and testicular tissues by considering different pathophysiological pathways. Male-control (MC), male-acrylamide (MACR), female-control (FC), and female-acrylamide (FACR) groups were formed. ACR was administered at a dose of 60 mg/kg for 5 days. ACR exposure decreased CAT and TrxR-specific activities, GSH levels, and Bcl-2 expression, while significantly increasing MDA, IL-6, and NFĸB p65 levels, caspase 3, and Bax expression in ovarian, uterine, and testicular tissues. Based on these results, it was determined that acrylamide induced damage in ovarian, uterine and testicular tissues through various pathways such as oxidative stress, inflammation, and apoptosis. Consequently, when selecting a therapeutic target, the substance whose efficacy is being investigated should be effective in these pathways. Furthermore, this study is the first to demonstrate the occurrence of bladder retention in both sexes following acrylamide exposure and will be an important step for future research.

Graphical abstract

查看原文本刊更多论文

丙烯酰胺暴露后卵巢、子宫和睾丸组织的生化和病理变化

丙烯酰胺（ACR）是一种受欢迎的物质，随着我们生活方式的改变，我们接触到的物质也越来越多，并带来了各种健康问题。为了确定针对ACR损伤的适当治疗方法，重要的是研究可能在其病理生理中有效的多种机制。本研究通过考虑不同的病理生理途径，探讨了ACR暴露对卵巢、子宫和睾丸组织的影响。形成雄性对照组（MC）、雄性丙烯酰胺组（MACR）、雌性对照组（FC）和雌性丙烯酰胺组（FACR）。ACR以60mg /kg的剂量给药，连续5天。ACR暴露降低了CAT和trxr特异性活性、GSH水平和Bcl-2表达，同时显著增加了卵巢、子宫和睾丸组织中MDA、IL-6和NFĸB p65水平、caspase 3和Bax表达。基于这些结果，我们确定丙烯酰胺通过氧化应激、炎症和细胞凋亡等多种途径引起卵巢、子宫和睾丸组织的损伤。因此，在选择治疗靶点时，所研究的物质应在这些途径中有效。此外，这项研究首次证明了丙烯酰胺暴露后男女都会出现膀胱潴留，这将是未来研究的重要一步。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.