Heterologous expression of a Synechocystis sp. PCC 6803 lipase gene in Synechococcus elongatus PCC 7942

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-05-27 DOI:10.1007/s10499-025-02052-2

Xiaotong Ren, Huaiyu Chen, Mengting Wang, Yongzhong Lu, Fang Wang

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引用次数: 0

Abstract

Lipases serve as valuable feed additives in aquaculture by enhancing lipid digestion and nutrient absorption. Recognizing the need for more efficient and sustainable enzyme sources, cyanobacterial lipases for their unique catalytic properties and environmental compatibility were explored. In this study, a lipase gene from Synechocystis sp. PCC 6803 was successfully expressed in Synechococcus elongatus PCC 7942 under the control of the endogenous Pcpc promoter. The recombinant strain showed ~ twofold higher lipase activity (p < 0.01) than wild-type, supported by increased protein expression on SDS-PAGE and enzymatic assays. When used as a feed additive for Synechogobius hasta, the transgenic algal supplement significantly improved growth performance, with a 21.7% increase in final body weight (p < 0.01) and a significantly lower feed conversion ratio (FCR) (p < 0.01) after 30 days of feeding compared to control groups. These findings suggest that the cyanobacterial lipase may serve as a potential feed additive that can enhance both nutritional uptake and growth performance in aquaculture.

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聚囊球菌pcc6803脂肪酶基因在长聚囊球菌pcc7942中的异源表达

脂肪酶通过促进脂质消化和营养吸收，在水产养殖中成为有价值的饲料添加剂。认识到需要更高效和可持续的酶源，蓝藻脂肪酶的独特催化性能和环境相容性进行了探索。本研究在内源性聚囊球菌Pcpc启动子的控制下，成功地在长聚囊球菌PCC 7942中表达了一个来自聚囊球菌PCC 6803的脂肪酶基因。重组菌株的脂肪酶活性比野生型高约2倍（p < 0.01）， SDS-PAGE和酶促检测结果显示重组菌株的蛋白表达增加。转基因藻添加物显著提高了黄颡鱼的生长性能，饲喂30 d后末重较对照组提高21.7% (p < 0.01)，饲料系数（FCR）显著降低（p < 0.01）。这些结果表明，蓝藻脂肪酶可能作为一种潜在的饲料添加剂，可以提高水产养殖的营养吸收和生长性能。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.