Real-time monitoring of Candida albicans biofilm growth and 4-HPA-mediated inhibition using an optoelectrochemical platform

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Anmol Kulshrestha, Pratima Gupta, Vivek Kumar Singh, Abhishek Kumar, Sonal Fande, Sanket Goel
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引用次数: 0

Abstract

The opportunistic fungus Candida albicans has become the major cause of hospital-acquired infections due to its ability to develop biofilms that resistance to treatments. his study monitored the real-time growth and inhibition of C. albicans hyphal and biofilm formation using optoelectrochemical approaches, focusing on Secreted Aspartyl Protease 5 (SAP5), a key virulence factor. The natural phenolic compound 4-Hydroxyphenylacetic acid (4-HPA) was computationally screened and demonstrated strong binding affinity to SAP5. In vitro studies indicated a minimum biofilm inhibitory concentration (MBIC) of 4 mg/mL and a minimum biofilm eradication concentration (MBEC) of 16 mg/mL. The 4-HPA exhibited considerable potential as an anti-hyphal and anti-biofilm agent, achieving efficacy > 90% in the Biofilm Infection Simulator System (BISS) platform. Likewise, in microfluidic platform, electrochemical analysis revealed 85–90% biofilm inhibition & reduction on MBIC & MBEC doses, respectively. The correlation of microscopic images with electrochemical data revealed complementarity, introducing a novel approach for monitoring microbial biofilms. This study introduces a novel approach to monitoring and treating biofilms, offering promising insights for anti-biofilm drug development.

Graphical abstract

利用光电电化学平台实时监测白色念珠菌生物膜生长和4- hpa介导的抑制作用
机会性真菌白色念珠菌已经成为医院获得性感染的主要原因,因为它能够形成生物膜,对治疗产生耐药性。他的研究利用光电化学方法实时监测了白色念珠菌菌丝的生长和抑制以及生物膜的形成,重点关注了关键毒力因子分泌天冬氨酸蛋白酶5 (SAP5)。通过计算筛选得到天然酚类化合物4-羟基苯基乙酸(4-HPA),发现其与SAP5具有较强的结合亲和力。体外研究表明,最低生物膜抑制浓度(MBIC)为4 mg/mL,最低生物膜根除浓度(MBEC)为16 mg/mL。4-HPA作为抗菌丝和抗生物膜剂显示出相当大的潜力,在生物膜感染模拟系统(BISS)平台上达到90%的有效性。同样,在微流控平台上,电化学分析显示85-90%的生物膜抑制作用;减少MBIC;分别为MBEC剂量。显微图像与电化学数据的相关性揭示了互补性,引入了一种监测微生物生物膜的新方法。本研究介绍了一种监测和治疗生物膜的新方法,为抗生物膜药物的开发提供了有希望的见解。图形抽象
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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