Diabetic wound healing via a co-delivery system for bone marrow derived stem cells and euphorbia hirta extract: an in vitro and in vivo study

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2025-05-26 DOI:10.1007/s10856-025-06880-9

Jiazhang Duan, Yun Yao, Jiafei Wang, Wuhua Liu, Saeed Rohani, Huagang Yang

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Abstract

Diabetes mellitus, a persistent metabolic disorder characterized by elevated blood glucose levels, presents a substantial global health challenge due to its escalating prevalence and associated complications. In the current study, a nanofibrous scaffold was developed for the co-delivery of bone marrow-derived stem cells and Euphorbia hirta extract into the diabetic wound beds. Scaffolds were produced using the electrospinning method, and their physicochemical and biological properties were studied in vitro. The wound healing function of the developed delivery system was investigated in a rat model of diabetic wound healing. The study showed that Euphorbia hirta extract-loaded scaffolds imparted no significant toxicity toward L929 cells and promoted their migration activity. A wound healing study showed that scaffolds loaded with both bone marrow-derived stem cells and euphorbia hirta extract had the highest wound healing activity, as shown by significantly higher wound closure percentage, collagen deposition, and epithelial thickness. Enzyme-linked immunosorbent Assay studies showed that these dressings reduced tissue oxidative stress and alleviated inflammatory responses.

Graphical Abstract

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通过骨髓干细胞和大戟提取物共同递送系统的糖尿病伤口愈合：一项体外和体内研究

糖尿病是一种以血糖水平升高为特征的持续性代谢紊乱，由于其不断上升的患病率和相关并发症，对全球健康构成了重大挑战。在目前的研究中，开发了一种纳米纤维支架，用于将骨髓来源的干细胞和大戟提取物共同递送到糖尿病伤口床。采用静电纺丝法制备支架，并对其体外理化和生物学性能进行了研究。在糖尿病大鼠创面愈合模型中研究了该递送系统的创面愈合功能。结果表明，大戟提取物负载支架对L929细胞无明显毒性，并能促进其迁移活性。一项伤口愈合研究表明，同时加载骨髓源性干细胞和大大麻提取物的支架具有最高的伤口愈合活性，伤口愈合率、胶原沉积和上皮厚度均显著提高。酶联免疫吸附试验研究表明，这些敷料减少了组织氧化应激，减轻了炎症反应。图形抽象

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.