Increased expression of membrane-bound TGF-β1, GITR, and GITR ligand in patients with autoimmune thyroid disease

IF 3.3 4区医学 Q3 IMMUNOLOGY

Immunology letters Pub Date : 2025-05-22 DOI:10.1016/j.imlet.2025.107036

Fumiaki Hayashi , Naoya Inoue , Yoshinori Iwatani , Yuka Yamashita , Hiroya Yamada , Akira Miyauchi , Mikio Watanabe

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Abstract

Regulatory T (Treg) cells, which play an important role in maintaining self-tolerance, are present in the thyroid-infiltrating lymphocytes of patients with autoimmune thyroid disease (AITD). We examined the expression of membrane-bound transforming growth factor-β1 (mTGF-β1), which mediates regulatory function and glucocorticoid-induced tumor necrosis factor receptor-related protein (GITR). The protein in turn may inhibit regulatory function on Treg cells and TGF-β1 receptor II (TGF-βRII) and GITR expression. We also evaluated GITR ligand (GITRL) localization in thyroid tissues. mTGF-β1⁺ cells proportion in Treg cells was higher in the thyroid of patients with AITD than in their peripheral blood. GITR⁺ cells proportion among Tregs and Teff cells was also higher in the thyroid than in peripheral blood. GITRL expression in thyrocytes was higher in AITD patients than in healthy subjects. The interaction and balance of mTGF-β1, GITR, TGF-βRII, and GITRL especially thyrocyte GITRL expression, could be critical in AITD pathogenesis.

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自身免疫性甲状腺疾病患者膜结合TGF-β1、GITR及GITR配体表达升高

自身免疫性甲状腺疾病（AITD）患者的甲状腺浸润淋巴细胞中存在调节T （Treg）细胞，在维持自身耐受性中起重要作用。我们检测了介导调节功能和糖皮质激素诱导的肿瘤坏死因子受体相关蛋白（GITR）的膜结合转化生长因子-β1 （mTGF-β1）的表达。该蛋白反过来可能抑制Treg细胞和TGF-β1受体II （TGF-βRII）以及GITR表达的调节功能。我们还评估了甲状腺组织中GITR配体（GITRL）的定位。AITD患者甲状腺组织中mTGF-β1+细胞在Treg细胞中的比例高于外周血。甲状腺组织中GITR+细胞在Tregs和Teff细胞中的比例也高于外周血。AITD患者甲状腺细胞中GITRL的表达高于健康人。mTGF-β1、GITR、TGF-βRII和GITRL尤其是甲状腺细胞GITRL表达的相互作用和平衡可能是AITD发病的关键。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunology letters 医学-免疫学

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

44 days

期刊介绍： Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.