Biocontrol effect of ginger glycoprotein and essential oil against Vicia faba damping-off caused by Fusarium solani and Rhizoctonia solani

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Kuwait Journal of Science Pub Date : 2025-05-23 DOI:10.1016/j.kjs.2025.100437

Hossam S. El-Beltagi , Ali Osman , Mahmoud Sitohy , Hayfa Habes Almutairi , Eman Eldesouky , Entsar Abbas , Ahmed Mahmoud Ismail , Hazem S. Elshafie , Ippolito Camele

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引用次数: 0

Abstract

The Fusarium solani and Rhizoctonia solani are destructive soil-borne pathogens worldwide, significantly impacting crop yields and quality. Synthetic fungicides are usually used for their control despite their adverse environmental and human health impacts. Therefore, there is a growing interest in discovering natural alternatives to synthetic pesticides. This study aims to assess the effectiveness of ginger glycoprotein and essential oil (EO) as antifungal agents against R. solani and F. solani, correlated with diseases that cause root decay and wilt in Vicia faba L. An in vitro trial assessed the antifungal efficacy of ginger EO and glycoprotein singularly (at 10 and 25 μg/mL) compared to Rizolex-T 50 WP (at 25, 50, and 75 μg/mL) against F. solani and R. solani mycelium growth. Whereas, the in vivo trial evaluated the efficacy of ginger EO and glycoprotein at 50 and 100 μg/mL, compared to Rizolex-T 50 WP at 50 μg/mL, in reducing pre- and post-emergence damping-off percentages. Sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR), High-performance liquid chromatography (HPLC), and particle size distribution were carried out to characterize seed glycoprotein. SDS-PAGE of the glycoprotein indicated two bands corresponding to 35 and 29 KDa. An HPLC examination of the glycoprotein carbohydrate component identified glucose, mannose, and fructose. GC-MS analysis of the components of EO identified 28 majors’ substances. R. solani and F. solani mycelial proliferation was dose-dependently inhibited by both EO and glycoprotein. Scanning Electron Microscope (SEM), used for investigating eventually morphological changes in tested fungi after treatment. In the in vivo test, it was seen that both EO and glycoprotein at 50 and 100 μg/mL made the symptoms caused by the tested fungi a lot less severe compared to the control. The results showed that ginger glycoprotein and essential oil might be able to be used instead of synthetic fungicides to treat V. faba damping off.

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生姜糖蛋白和精油对枯萎菌和枯丝核菌致蚕豆枯萎病的防效研究

枯萎菌和枯丝核菌是世界范围内具有破坏性的土传病原体，严重影响作物产量和品质。尽管合成杀菌剂对环境和人类健康有不利影响，但它们通常被用于控制它们。因此，人们对寻找合成农药的天然替代品越来越感兴趣。本研究旨在评价生姜糖蛋白和精油（EO）对蚕豆根腐病和枯萎病相关病原菌solani和F. solani的抑菌效果。通过体外试验，比较单独使用生姜糖蛋白和糖蛋白（10和25 μg/mL）与rizolx - t50 WP（25、50和75 μg/mL）对F. solani和R. solani菌丝体生长的抑菌效果。然而，体内试验评估了50和100 μg/mL的生姜EO和糖蛋白的效果，与50 μg/mL的利唑列- t50 WP相比，降低了出现前和出现后的衰减百分比。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）、傅里叶变换红外光谱（FTIR）、高效液相色谱（HPLC）和粒径分布等方法对种子糖蛋白进行表征。糖蛋白的SDS-PAGE显示了35和29 KDa对应的两条条带。用高效液相色谱法检测糖蛋白碳水化合物成分，鉴定出葡萄糖、甘露糖和果糖。气相色谱-质谱分析鉴定出28种主要成分。EO和糖蛋白均能呈剂量依赖性地抑制茄蚜和茄蚜菌丝的增殖。扫描电子显微镜（SEM），用于研究被试真菌在处理后的最终形态变化。在体内试验中发现，50和100 μg/mL的EO和糖蛋白均使被试真菌引起的症状比对照组轻得多。结果表明，生姜糖蛋白和生姜精油可以代替合成杀菌剂防治蚕豆枯萎病。

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来源期刊

Kuwait Journal of Science MULTIDISCIPLINARY SCIENCES-

CiteScore

1.60

自引率

28.60%

发文量

132

期刊介绍： Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.