Rhizobium binxianense sp. nov. and Rhizobium mulingense sp. nov., isolated from nodules of Phaseolus vulgaris in Heilongjiang Province of China

IF 4.2 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-05-20 DOI:10.1016/j.syapm.2025.126619

Yuxi Liu , Lili Liu , Ziqi Wang , Xiaoqian Huang , Yuxin Dong , Dong Hu , Chuntao Gu , En Tao Wang , Hao Wang

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引用次数: 0

Abstract

Rhizobial isolates from Phaseolus vulgaris (common bean) grown in Heilongjiang Province were grouped into two clusters based upon the phylogenies of 16S rRNA and recA-atpD genes representing by BJ04^T, MJ22, MJ37, BC56, MC62 and MC63^T, MJ21, MJ31, respectively. The phylogenetic analysis of whole genomes further revealed that BJ04^T, MJ22, MJ37, BC56 and MC62 formed a cluster neighbored Rhizobium chutanense C5^T, while MC63^T, MJ21 and MJ31 formed a cluster most related with Rhizobium croatiense 13T^T. The whole genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the strains BJ04^T and R. chutanense C5^T and between MC63^T and R. croatiense 13T^T were lower than the species thresholds 95 % and 70 %, respectively. While the G + C content of the whole genome of the novel strains were 61.16–61.56 %, within the allowable GC content range of the Rhizobium genus (57–66 %). These results, combined with chemical classification and phenotype analysis, supported that BJ04^T and MC63^T represented two novel species and the names Rhizobium binxianense sp. nov. and Rhizobium mulingense sp. nov. were described for them. BJ04^T (=CCTCC AB 2022367^T, = JCM 35885^T) and MC63^T (=CCTCC AB 2024025^T, = JCM 36652^T) were designed as the type strains, respectively for these two novel species.

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从黑龙江省菜豆根瘤中分离的滨咸根瘤菌和mulingense根瘤菌

根据16S rRNA和recA-atpD基因（分别为BJ04T、MJ22、MJ37、BC56、MC62和MC63T、MJ21、MJ31）的系统发育特征，将黑龙江省菜豆根瘤菌分离物分为两类。全基因组系统发育分析进一步表明，BJ04T、MJ22、MJ37、BC56和MC62与chuutanense C5T形成一个集群，而MC63T、MJ21和MJ31与克罗地亚根瘤菌13TT形成一个集群。菌株BJ04T与楚坦纳河鼠C5T、MC63T与克罗地亚纳河鼠13TT的全基因组平均核苷酸同源性（ANI）和数字DNA-DNA杂交（dDDH）值分别低于种间阈值95%和70%。新菌株全基因组G + C含量为61.16 ~ 61.56%，在根瘤菌属允许的GC含量范围（57 ~ 66%）内。这些结果结合化学分类和表型分析，支持BJ04T和MC63T为两个新种，并为它们命名为binxianense sp. 11和mulingense sp. 11。将BJ04T （=CCTCC AB 2022367T, = JCM 35885T）和MC63T （=CCTCC AB 2024025T, = JCM 36652T）分别设计为这两个新种的型菌株。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: