A highly sensitive and flexible triple-modal multiplex immunoassay for on-site screening of illegal anti-inflammatory drugs in herbal teas

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-05-26 DOI:10.1016/j.snb.2025.138046

Maner Chen , Mingtong Guan , Guangyao Li , Xiaoqin Yu , Jiaxin Wen , Yi Lei , Hongtao Lei , Tian Guan

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引用次数: 0

Abstract

Rapid, convenient and multi-modal lateral flow immunochromatographic assays (LFIAs) are encouraging and preferable in food safety detection. Herein, based on the easily available, photo-heat sensitive natural cuttlefish ink nanoparticles (CINPs) and strongly fluorescent AIE microspheres, a colorimetric/fluorescence/photothermal triple-modal multiplex LFIA was developed for the rapid determination of three prohibited inflammatory drugs in herbal teas. Due to the inner filter effect between CINPs-labeled antibodies and AIE microspheres-antigens, this smartphone-based portable biosensor yielded high sensitivities toward 4-acetamidophenol, phenacetin, and prednisone acetate (1.75, 2.60, and 0.99 ng/mL in colorimetric modal; 1.52, 1.90, and 0.29 ng/mL in fluorescence modal; 0.67, 1.73, and 0.21 ng/mL in photothermal modal), respectively. Moreover, reasonable recoveries (89.46 - 118.96 %), acceptable coefficients of variation (2.30 % - 11.2 %) and parallel results with authorized LC-MS/MS in recovery test as well as blind samples analysis verified the high reliability and applicability of the proposed work. This work highlights a flexible, simple, low-cost yet accurate LFIA in the field of food safety detection, which can also be extended to the environmental monitoring and medical diagnostics.

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一种高灵敏、灵活的三模态多重免疫分析法用于草药茶中非法抗炎药物的现场筛选

快速、方便、多模态的横向流动免疫层析法（LFIAs）在食品安全检测中具有广泛的应用前景。本文以光热敏天然墨鱼墨水纳米颗粒（CINPs）和强荧光AIE微球为基础，建立了一种比色/荧光/光热三模态多重LFIA，用于草药茶中三种禁用炎症药物的快速检测。由于cinps标记的抗体和AIE微球抗原之间的内部过滤效应，这种基于智能手机的便携式生物传感器对4-乙酰氨基酚、非那西丁和醋酸泼尼松具有高灵敏度（比色模式为1.75、2.60和0.99 ng/mL）；荧光模态分别为1.52、1.90和0.29 ng/mL；分别为0.67、1.73和0.21 ng/mL（光热模态）。合理回收率（89.46 ~ 118.96%）、可接受变异系数（2.30% ~ 11.2%）、与授权LC-MS/MS的回收率及盲样分析结果平行，验证了所提方法具有较高的可靠性和适用性。这项工作突出了一种灵活、简单、低成本、准确的LFIA在食品安全检测领域的应用，并可扩展到环境监测和医疗诊断领域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.