Nuclear magnetic resonance-based metabolomic study of rat brain after different intensity treadmill running.

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ni Zeng, Jie-Ting Li, Zhi-Juan Zhang, Zhi-Peng Yan, Tao Liao, Guo-Xin Ni
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Abstract

Previous studies have revealed that different intensities of exercise training have an impact on cognition. However, the cognitive effects of different intensities of exercise and its underlying mechanisms are not fully understood. The aim of this paper was to investigate the effects of different intensities of treadmill exercise on cognition in rats from the perspective of metabolomic analysis. In this study, ninety-six male rats were randomly divided into four groups: control group (CON group, n = 24), low-intensity running group (LIR group, n = 24), medium-intensity running group (MIR group, n = 24), and high-intensity running group (HIR group, n = 24). After 4 weeks of treadmill running, rats in the LIR group located the platform significantly faster than those in the CON(p = 0.027) and HIR(p = 0.011) groups. After 8 weeks of treadmill running, rats in the LIR and MIR groups also found the platform more quickly than those in CON group (p = 0.003 and p = 0.015, respectively). Additionally, rats in the MIR group showed significantly increased superoxide dismutase (SOD)/catalase (CAT) in the hippocampus compared with those exposed to HIR(p = 0.03), LIR(p = 0.0008), and CON(p = 0.0004). Metabolomic analysis revealed that, after 8 weeks of running, 14 metabolites with similar characteristics differed between the MIR and HIR groups compared to the CON group. The LIR group showed significant alterations in 12 key metabolites compared to the CON group. The LIR, MIR, and HIR groups also demonstrated significant changes in 3, 4, and 3 metabolic pathways respectively, when compared to the CON group. In conclusion, the above results indicate that LIR can effectively decrease fumarate accumulation, thereby enhancing the TCA cycle and brain energy metabolism which in turn improved cognitive function, while MIR can modify glutathione metabolism to alleviate oxidative stress (OS), supporting cognitive function.

基于核磁共振的不同强度跑步机运动后大鼠脑代谢组学研究。
先前的研究表明,不同强度的运动训练对认知有影响。然而,不同强度的运动对认知的影响及其潜在机制尚不完全清楚。本文旨在从代谢组学的角度探讨不同强度的跑步机运动对大鼠认知能力的影响。将96只雄性大鼠随机分为4组:对照组(CON组,n = 24)、低强度跑步组(LIR组,n = 24)、中强度跑步组(MIR组,n = 24)、高强度跑步组(HIR组,n = 24)。在跑步4周后,LIR组大鼠定位平台的速度明显快于CON组(p = 0.027)和HIR组(p = 0.011)。在跑步8周后,LIR组和MIR组的大鼠发现平台的速度也比CON组快(p = 0.003和p = 0.015)。此外,MIR组大鼠海马超氧化物歧化酶(SOD)/过氧化氢酶(CAT)显著高于HIR组(p = 0.03)、LIR组(p = 0.0008)和CON组(p = 0.0004)。代谢组学分析显示,跑步8周后,MIR组和HIR组与CON组相比,有14种具有相似特征的代谢物存在差异。与CON组相比,LIR组在12个关键代谢物中表现出显著改变。与CON组相比,LIR、MIR和HIR组在3、4和3条代谢途径上也分别表现出显著的变化。综上所述,LIR可有效减少富马酸盐积累,从而增强TCA循环和脑组织能量代谢,进而改善认知功能;MIR可调节谷胱甘肽代谢,缓解氧化应激,支持认知功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of physiology and biochemistry
Journal of physiology and biochemistry 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiology and Biochemistry publishes original research articles and reviews describing relevant new observations on molecular, biochemical and cellular mechanisms involved in human physiology. All areas of the physiology are covered. Special emphasis is placed on the integration of those levels in the whole-organism. The Journal of Physiology and Biochemistry also welcomes articles on molecular nutrition and metabolism studies, and works related to the genomic or proteomic bases of the physiological functions. Descriptive manuscripts about physiological/biochemical processes or clinical manuscripts will not be considered. The journal will not accept manuscripts testing effects of animal or plant extracts.
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