A Novel Strawberry Disease Associated With Leaf Spot, Crown Rot, and Root Rot Caused by Neopestalotiopsis rosae in Italy

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2025-05-25 DOI:10.1111/jph.70071

Stefania Mirela Mang, Ippolito Camele, Carmine Palmieri, Carmine Marcone

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引用次数: 0

Abstract

In the spring of 2023 in Eboli and Caserta (Campania, southern Italy), strawberry plants (var. Marimbella) grown in organic open fields showed an outbreak of a severe and unprecedented decline (disease incidence reaching > 80%) associated with root rot, crown rot, and leaf spot and closely resembling symptoms reported previously in other countries for Neopestalotiopsis spp. infection. Therefore, the present study was undertaken with the aim of determining the aetiology of this serious disease. Fungal isolates were obtained from symptomatic strawberry plants and investigated in detail for molecular identification. Phylogenetic analysis was conducted by amplifying and sequencing three DNA barcodes: the internal transcribed spacer (ITS) region of rDNA, the β-tubulin (tub2) partial gene, and the translation elongation factor 1α (tef1) partial gene. Symptoms observed in the field were replicated in pathogenicity tests, conducted by inoculating strawberry (var. Marimbella) leaves, fruits and plants, thus satisfying Koch's postulates. Phylogenetic analyses identified the causal agent as Neopestalotiopsis rosae. To our knowledge, this is the first report of the emerging and serious fungal pathogen N. rosae infecting strawberry in Italy.

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意大利一种新的草莓病害，与新拟盘多毛孢引起的叶斑病、冠腐病和根腐病相关

2023年春，在Eboli和Caserta（意大利南部坎帕尼亚），种植在有机露天田里的草莓（Marimbella品种）爆发了一场前所未有的严重衰退（发病率达到80%），与根腐病、冠腐病和叶斑病有关，与其他国家以前报道的新estestalotiopsis spp感染症状非常相似。因此，本研究的目的是确定这一严重疾病的病因。从有症状的草莓植株中分离得到真菌，并对其进行了详细的分子鉴定。通过扩增rDNA内部转录间隔区（ITS）、β-微管蛋白（tub2）部分基因和翻译延伸因子1α (tef1)部分基因三个DNA条形码进行系统发育分析和测序。通过接种草莓（Marimbella品种）的叶子、果实和植株进行致病性试验，在田间观察到的症状在致病性试验中得到了重复，从而满足了Koch的假设。系统发育分析鉴定病原为新拟盘多毛孢。据我们所知，这是意大利首次报道草莓中出现的严重真菌病原体玫瑰孢杆菌。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.