Enhancement of ethanol production using CO2 and CO gases in a continuous fixed-film bioreactor

IF 7.2 2区工程技术 Q1 CHEMISTRY, MULTIDISCIPLINARY

Journal of CO2 Utilization Pub Date : 2025-05-26 DOI:10.1016/j.jcou.2025.103133

Siraporn Potivichayanon , Prayad Pokethitiyook , Rattana Toensakes

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Abstract

Ethanol-producing bacteria play an essential role in enhancing bioethanol production efficiency. This study aimed to develop an application of Petrobacter sp. SUTSP5, an ethanol-producing bacterium, from carbon-rich gas by using a fixed-film ethanol bioreactor called FEthaRex to enhance ethanol production efficiency.

The fixed-film consortium was initially grown in a combination nutrient with a carbon-rich gas composed of 15:25:40 (by volume) CO₂:CO:H₂ and supplied to the FEthaRex column at an average flow rate of 100 mL/min. Nutritional liquid medium was circulated at a flow rate of 20 mL/min. The system achieved an ethanol production rate of approximately 0.1305 g/L/h within 24 h, which increased significantly to 0.1780 g/L/h after a 48-h operation period; the ethanol production rate increased from 0.1585–0.2132 g/L/h as the gas composition increased to 30:50:20 (by volume); continuous operation of the FEthaRex system was achieved after mixed gas feeding was stopped; and the maximum ethanol concentration observed after 48 h was 1.9860 g/L/h, or 6.0411 % by volume, with the ethanol-producing cells reaching a count of approximately 10⁷ CFU/mL and average biofilm cell attached to packing media roughly 0.0058 g. At these optimum conditions, which achieved an ethanol yield higher than that of a free suspended cell reactor (EthaRex) with the same gas feed conditions. The FEthaRex system was demonstrated as a promising approach to achieving cell density and stability in the microbial community. This technology could be an efficient tool for enhancing sustainable bioethanol production.

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在连续固定膜生物反应器中利用CO2和CO气体提高乙醇生产

产乙醇菌在提高生物乙醇生产效率方面发挥着重要作用。本研究旨在利用固定膜乙醇生物反应器FEthaRex，从富碳气体中提取乙醇生产细菌Petrobacter sp. SUTSP5，以提高乙醇生产效率。固定膜联盟最初在一种由15:25:40（体积）CO2:CO:H2组成的富碳气体的组合营养物中生长，并以100 mL/min的平均流速供应给FEthaRex柱。营养液培养基以20 mL/min的流速循环。在24 h内，系统的乙醇产率约为0.1305 g/L/h，运行48 h后，系统的乙醇产率显著提高至0.1780 g/L/h；当气体组成增加到30:50:20（体积比）时，乙醇产率从0.1585 ~ 0.2132 g/L/h增加；停止混合气体进料后，实现FEthaRex系统的连续运行；48 h后观察到的最大乙醇浓度为1.9860 g/L/h，占体积的6.0411 %，产乙醇细胞的计数约为107 CFU/mL，平均附着在包装介质上的生物膜细胞约为0.0058 g。在此条件下，乙醇的产率高于相同进气条件下的自由悬浮细胞反应器（EthaRex）。FEthaRex系统被证明是一种很有前途的方法，可以在微生物群落中实现细胞密度和稳定性。该技术可能是提高可持续生物乙醇生产的有效工具。

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来源期刊

Journal of CO2 Utilization CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL

CiteScore

13.90

自引率

10.40%

发文量

406

审稿时长

2.8 months

期刊介绍： The Journal of CO2 Utilization offers a single, multi-disciplinary, scholarly platform for the exchange of novel research in the field of CO2 re-use for scientists and engineers in chemicals, fuels and materials. The emphasis is on the dissemination of leading-edge research from basic science to the development of new processes, technologies and applications. The Journal of CO2 Utilization publishes original peer-reviewed research papers, reviews, and short communications, including experimental and theoretical work, and analytical models and simulations.