Trace protein detection by graphene-integrated terahertz meta-sensor and enhanced sensitivity using gold nanoparticles-assisted

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-20 DOI:10.1016/j.bios.2025.117612

Tongling Wang , Ziqi Li , Mingyao Wang , Changshun Wu , Maosheng Yang , Xiuwei Yang , Li Wang , Kuilong Li , Zhou Yang , Wenjing Zheng , Lanju Liang , Haiyun Yao , Xin Yan , Jianquan Yao

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引用次数: 0

Abstract

With the rapid development of terahertz (THz) biosensing technology, the research on trace protein detection via THz metasurfaces has attracted significant attention. Here, we propose a graphene-integrated toroidal split-ring resonator (TSRR) THz meta-sensor for detecting protein at trace levels based on the Dirac property of chemical vapor deposition (CVD) graphene. The experimental results show that the designed meta-sensor can realize the detection of midkine with a 125 pg/mL limit of detection (LOD). Furthermore, benefiting from the charge accumulation generated by the local surface plasmon resonance of gold nanoparticles (AuNPs) to induce the enhancement of the local electric field of graphene, trace carcinoembryonic antigen (CEA) detection with an LOD of 10 pg/mL is achieved assisted by CEA antibody-AuNPs. The proposed meta-sensor realizes picogram-level protein detection, providing new ideas for applying metasurface-based THz sensors in biosensing.

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石墨烯集成太赫兹元传感器检测痕量蛋白质及金纳米粒子辅助增强灵敏度

随着太赫兹（THz）生物传感技术的迅速发展，利用太赫兹超表面检测微量蛋白质的研究受到了广泛关注。在这里，我们提出了一种基于化学气相沉积（CVD）石墨烯的狄拉克特性的石墨烯集成环形裂环谐振器（TSRR）太赫兹元传感器，用于检测微量水平的蛋白质。实验结果表明，所设计的元传感器可实现对midkine的检测，检测限为125 pg/mL。此外，利用金纳米粒子（AuNPs）的局部表面等离子体共振产生的电荷积累，诱导石墨烯的局部电场增强，CEA抗体-AuNPs辅助检测微量癌胚抗原（CEA）， LOD为10 pg/mL。该传感器实现了皮图级蛋白质检测，为超表面太赫兹传感器在生物传感中的应用提供了新的思路。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.