The effects of supplemental brewers yeast on postprandial amino acid concentrations in healthy adult sled dogs

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
L M Rummell, J R Templeman, C L Cargo-Froom, A K Shoveller
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引用次数: 0

Abstract

Yeast has been used in animal systems to modulate the immune response and support gut health. Certain amino acids (AA) are reported to also exert positive effects on the gut, supporting the intestinal barrier and restoring mucosal immune homeostasis. The objective of this study was to evaluate the effects of brewers yeast on postprandial serum AA appearance in dogs. Nineteen privately-owned domestic Siberian huskies and one Alaskan husky (9 females: 5 intact, 4 spayed; 11 males: 3 intact, 8 neutered), with an average age of 4.8 ± 2.6 years and body weight of 25.6 ± 4.1 kg, were enrolled in this study. Ten dogs received a dry extruded control diet (Ctl) and ten the Ctl diet top-dressed with yeast to receive a daily ß–glucan dose of 7 mg/kg BW (treatment, Trt) for 10 weeks. At weeks -1, 2, 4, and 8 postprandial free AA concentrations were quantified. A fasted blood sample was collected, a meal was provided, followed by further blood sampling 1, 2, and 4 h post-meal. Serum IL-10 concentrations were analyzed from the fasted sample. All data were analyzed using PROC GLIMMIX of SAS, with dog as a random effect and week, diet group, and sampling time point as fixed effects for AA, and with dog as a random effect and week as a fixed effect and repeated measure for IL-10. There was no effect of treatment on any AA, though a significant Trt*wk*timepoint interaction effect was observed for His and Trp (P ≤ 0.05). An overall increase was observed in many AAs for all dogs – serum concentrations of Lys, Met, Thr, Trp, Ala, Arg, Gln, Gly, Pro, Ser, Tau, and Tyr were greatest at week 8 in all dogs when compared to concentrations at week -1 (P ≤ 0.05). Serum IL-10 concentrations did not differ by week or between groups (P > 0.05). In three Trt dogs that had greater gut permeability than all other dogs at week -2, as reported in a previous publication, serum Ile, Thr, and Val concentrations were greater at week 8 compared to week -1 (P ≤ 0.05) and Leu concentrations were greater 1 and 2 h postprandial at week 8 compared to the same time points at week -1 (P ≤ 0.05). The results of this study suggest that supplemental yeast may have a beneficial effect on peripheral AA availability without impacting inflammatory status in dogs. Future studies should seek to investigate the effects of reducing or improving gut permeability with yeast on nutrient digestive and metabolic efficiencies or consider yeast in clinical nutrition to support dogs with gastrointestinal diseases.
添加啤酒酵母对健康成年雪橇犬餐后氨基酸浓度的影响
酵母已被用于动物系统调节免疫反应和支持肠道健康。据报道,某些氨基酸(AA)也对肠道发挥积极作用,支持肠道屏障和恢复粘膜免疫稳态。本研究的目的是评估啤酒酵母对狗餐后血清AA外观的影响。19只私人拥有的家养西伯利亚哈士奇和1只阿拉斯加哈士奇(9只雌性:5只完整,4只绝育;雄性11只,完整3只,绝育8只,平均年龄4.8±2.6岁,体重25.6±4.1 kg。10只狗饲喂干挤压对照饲粮(Ctl)和10只Ctl饲粮加酵母,每天给予7 mg/kg BW的ß -葡聚糖(Trt)治疗,持续10周。在第1、2、4和8周时,定量测定餐后游离AA浓度。采集空腹血样,提供膳食,然后在餐后1、2和4小时进一步采血。从禁食样品中分析血清IL-10浓度。所有数据采用SAS的PROC GLIMMIX进行分析,AA以犬为随机效应,周、日粮组和采样时间点为固定效应;IL-10以犬为随机效应,周为固定效应,重复测量。治疗对任何AA均无影响,但His和Trp存在显著的Trt*工时*时间点交互作用(P≤0.05)。与第1周相比,所有犬的血清中Lys、Met、Thr、Trp、Ala、Arg、Gln、Gly、Pro、Ser、Tau和Tyr的浓度在第8周时最高(P≤0.05)。血清IL-10浓度在周间或组间无差异(P >;0.05)。在之前的一篇文章中,有3只Trt犬在第2周肠道通透性高于其他所有犬,血清Ile、Thr和Val浓度在第8周高于第1周(P≤0.05),而Leu浓度在第8周餐后1和2小时高于第1周的同一时间点(P≤0.05)。本研究结果表明,添加酵母可能对狗的外周AA利用率有有益影响,而不会影响炎症状态。未来的研究应寻求调查酵母降低或提高肠道通透性对营养物质消化和代谢效率的影响,或考虑酵母在临床营养中支持患有胃肠道疾病的狗。
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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