Host genome drives the microbiota enrichment of beneficial microbes in shrimp: exploring the hologenome perspective.

IF 4.9 Q1 MICROBIOLOGY
Fernanda Cornejo-Granados, Luigui Gallardo-Becerra, Sandra Romero-Hidalgo, Alonso A Lopez-Zavala, Andrés Cota-Huízar, Melany Cervantes-Echeverría, Rogerio R Sotelo-Mundo, Adrian Ochoa-Leyva
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引用次数: 0

Abstract

Background: Pacific Whiteleg shrimp (Litopenaeus vannamei) is an important model for breeding programs to improve global aquaculture productivity. However, the interaction between host genetics and microbiota in enhancing productivity remains poorly understood. We investigated the effect of two shrimp genetic lines, Fast-Growth (Gen1) and Disease-Resistant (Gen2), on the microbiota of L. vannamei.

Results: Using genome-wide SNP microarray analysis, we confirmed that Gen1 and Gen2 represented distinct genetic populations. After confirming that the rearing pond did not significantly influence the microbiota composition, we determined that genetic differences explained 15.8% of the microbiota variability, with a stronger selective pressure in the hepatopancreas than in the intestine. Gen1, which exhibited better farm productivity, fostered a microbiota with greater richness, diversity, and resilience than Gen2, along with a higher abundance of beneficial microbes. Further, we demonstrated that a higher abundance of beneficial microbes was associated with healthier shrimp vs. diseased specimens, suggesting that Gen1 could improve shrimp's health and productivity by promoting beneficial microbes. Finally, we determined that the microbiota of both genetic lines was significantly different from their wild-type counterparts, suggesting farm environments and selective breeding programs strongly alter the natural microbiome.

Conclusions: This study highlights the importance of exploring the hologenome perspective, where integrating host genetics and microbiome composition can enhance breeding programs and farming practices.

宿主基因组驱动虾有益微生物的微生物群富集:探索全基因组视角。
背景:凡纳滨对虾(Litopenaeus vannamei)是提高全球水产养殖生产力的重要养殖模式。然而,宿主遗传学和微生物群在提高生产力方面的相互作用仍然知之甚少。研究了快速生长对虾(Gen1)和抗病对虾(Gen2)两个遗传品系对凡纳美对虾(l.s annamei)菌群的影响。结果:使用全基因组SNP微阵列分析,我们证实Gen1和Gen2代表不同的遗传群体。在确认饲养池对微生物群组成没有显著影响后,我们确定遗传差异解释了15.8%的微生物群变异,肝胰腺中的选择压力比肠道中的更强。Gen1表现出更好的农业生产力,培养了比Gen2更丰富、多样性和弹性的微生物群,以及更高丰度的有益微生物。此外,研究人员还发现,与患病虾相比,健康虾的有益微生物丰度更高,这表明Gen1可以通过促进有益微生物来改善虾的健康和生产力。最后,我们确定这两个遗传系的微生物群与野生型的微生物群显著不同,这表明农场环境和选择性育种计划强烈地改变了天然微生物群。结论:本研究强调了探索全基因组视角的重要性,整合宿主遗传学和微生物组组成可以提高育种计划和农业实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
0.00%
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审稿时长
13 weeks
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