Antibacterial and Antibiofilm Efficacy of Green Synthesised Haloxylon Capped Silver Nanoparticles Against Enterococcus faecalis.

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2025-05-22 DOI:10.1111/aej.12948

Rawda O Alsuwaidi, Priyadharshini Sekar, Abdelaziz Elgamouz, Shashikant P Patole, Alaa Alatta, Anil Kishen, Venkateshbabu Nagendrababu

{"title":"Antibacterial and Antibiofilm Efficacy of Green Synthesised Haloxylon Capped Silver Nanoparticles Against Enterococcus faecalis.","authors":"Rawda O Alsuwaidi, Priyadharshini Sekar, Abdelaziz Elgamouz, Shashikant P Patole, Alaa Alatta, Anil Kishen, Venkateshbabu Nagendrababu","doi":"10.1111/aej.12948","DOIUrl":null,"url":null,"abstract":"<p><p>This study synthesised and characterised Haloxylon-capped silver nanoparticles (Haloxylon-AgNPs) and assessed their antibacterial and antibiofilm activity against Enterococcus faecalis. The study also examined Haloxylon-AgNPs' ability to target E. faecalis biofilms in root canals. Haloxylon salicornicum extract was used to synthesise silver nanoparticles. Physiochemical characterisation of Haloxylon-AgNPs was conducted. Antimicrobial and antibiofilm activity of Haloxylon-AgNPs was studied by agar diffusion method, minimum inhibitory concentration (MIC) determination, time-kill assay, confocal laser scanning microscopy (CLSM) and qPCR for virulence gene attenuation. One hundred and twenty extracted teeth were infected with E. faecalis and treated with either Haloxylon-AgNPs, chlorhexidine, calcium hydroxide or saline. The data were analysed using a one-way ANOVA and Tukey multiple comparison test to investigate the bacterial reduction between groups (p < 0.05, significant; p < 0.001, highly significant). A significant reduction in the thickness of biofilm and expression of cylA virulence gene of Haloxylon-AgNPs treated E. faecalis was observed. No difference was observed between Haloxylon-AgNPs, chlorhexidine and calcium hydroxide in the tooth model.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Endodontic Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/aej.12948","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

This study synthesised and characterised Haloxylon-capped silver nanoparticles (Haloxylon-AgNPs) and assessed their antibacterial and antibiofilm activity against Enterococcus faecalis. The study also examined Haloxylon-AgNPs' ability to target E. faecalis biofilms in root canals. Haloxylon salicornicum extract was used to synthesise silver nanoparticles. Physiochemical characterisation of Haloxylon-AgNPs was conducted. Antimicrobial and antibiofilm activity of Haloxylon-AgNPs was studied by agar diffusion method, minimum inhibitory concentration (MIC) determination, time-kill assay, confocal laser scanning microscopy (CLSM) and qPCR for virulence gene attenuation. One hundred and twenty extracted teeth were infected with E. faecalis and treated with either Haloxylon-AgNPs, chlorhexidine, calcium hydroxide or saline. The data were analysed using a one-way ANOVA and Tukey multiple comparison test to investigate the bacterial reduction between groups (p < 0.05, significant; p < 0.001, highly significant). A significant reduction in the thickness of biofilm and expression of cylA virulence gene of Haloxylon-AgNPs treated E. faecalis was observed. No difference was observed between Haloxylon-AgNPs, chlorhexidine and calcium hydroxide in the tooth model.

查看原文本刊更多论文

绿色合成氟氧ylon包覆银纳米颗粒对粪肠球菌的抗菌和抗生物膜效果。

本研究合成并表征了Haloxylon-AgNPs纳米银微粒（Haloxylon-AgNPs），并评估了它们对粪肠球菌的抗菌和抗生物膜活性。该研究还检测了Haloxylon-AgNPs靶向根管中粪肠杆菌生物膜的能力。以盐角兰提取物为原料合成纳米银。对卤代酮agnps进行了理化表征。采用琼脂扩散法、最低抑菌浓度（MIC）测定、时间杀伤法、共聚焦激光扫描显微镜（CLSM）和毒力基因衰减qPCR等方法研究了Haloxylon-AgNPs的抑菌活性和抗生物膜活性。120颗拔除的牙齿感染粪肠杆菌，分别用Haloxylon-AgNPs、氯己定、氢氧化钙或生理盐水处理。采用单因素方差分析和Tukey多重比较检验对数据进行分析，以调查组间细菌减少的情况(p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.