Hepatoprotective effects of dandelion against AFB1-induced liver injury are associated with activation of bile acid-FXR signaling in chicks

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-05-20 DOI:10.1016/j.toxicon.2025.108419

Zhe Peng , Jiang Deng , Ze-Jing Xu , Qin-Jian Niu , Lamesgen Dessalegn , Alainaa Refaie , Lv-Hui Sun , Yan-Ping Feng , Meng Liu

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引用次数: 0

Abstract

This study aimed to investigate the protective effects of dandelion against AFB₁-induced hepatotoxicity through the regulation of the FXR signaling pathway in chicks. A total of 144 one-day-old male broilers were randomly assigned to three groups and received a basal diet (BD), and BD supplemented with 0.5 mg/kg of AFB₁ or 0.5 mg/kg AFB₁ with 0.4 % dandelion for 3 weeks. The results showed that the AFB₁ treatment caused liver injury and decreased the concentrations of albumin and alkaline phosphatase in serum and increased the total bile acid concentration in serum and liver. Dietary AFB₁ supplementation also induced hepatocyte swelling, necrosis, neutrophils infiltration and lipid deposition in the liver. Notably, dietary dandelion supplementation alleviated these alterations induced by AFB₁. Additionally, dietary dandelion supplementation alleviated AFB₁-induced changes in ileum microbiota and decreased the abundance of Lactobacillus, L. vaginalis, and L. acidophilus compared to the AFB₁ treatment. Furthermore, AFB₁ downregulated Baat, Ntcp, Acc, FXR, SHP, and SREBP-1c expression, and upregulated Cyp8b1, Bacs, Fas, Pparα, Lxrα and CYP7A1 expression in liver. Meanwhile, AFB₁ also downregulated Fgf19, Ostα, Ostβ and FXR expression and upregulated SHP expression in the ileum. Conclusively, dietary dandelion supplementation protected broilers from AFB₁-induced hepatotoxicity, potentially due to the activation of bile acid-FXR signaling pathway.

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蒲公英对afb1诱导的雏鸡肝损伤的保护作用与激活胆汁酸- fxr信号有关。

本研究旨在探讨蒲公英通过调控雏鸡FXR信号通路对afb1诱导的肝毒性的保护作用。试验选用144只1日龄肉鸡，随机分为3组，分别饲喂基础饲粮（BD）和在基础饲粮中添加0.5 mg/kg AFB1或0.5 mg/kg AFB1中添加0.4%蒲公英，试验持续3周。结果表明，AFB1处理引起大鼠肝损伤，血清白蛋白和碱性磷酸酶浓度降低，血清和肝脏总胆汁酸浓度升高。饲粮中添加AFB1还会引起肝细胞肿胀、坏死、中性粒细胞浸润和肝脏脂质沉积。值得注意的是，饲粮中添加蒲公英可减轻AFB1诱导的这些改变。此外，与AFB1处理相比，饲粮中添加蒲公英减轻了AFB1诱导的回肠微生物群变化，降低了乳酸菌、阴道乳杆菌和嗜酸乳杆菌的丰度。此外，AFB1下调肝脏Baat、Ntcp、Acc、FXR、SHP和SREBP-1c的表达，上调Cyp8b1、Bacs、Fas、Pparα、Lxrα和CYP7A1的表达。同时，AFB1下调回肠中Fgf19、Ostα、Ostβ和FXR的表达，上调SHP的表达。总之，饲粮中添加蒲公英可以保护肉仔鸡免受afb1诱导的肝毒性，可能是由于激活了胆汁酸- fxr信号通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

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