Variation in diet concentration and bacterial inoculum size in larval habitats shapes the performance of the Asian tiger mosquito, Aedes albopictus.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY
Vincent Raquin, Edwige Martin, Guillaume Minard, Claire Valiente Moro
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引用次数: 0

Abstract

Background: Ecological niches present unique environmental and biological trademarks such as abiotic conditions, nutrient availability, and trophic interactions that may impact the ecology of living organisms. Female mosquitoes deposit their eggs in aquatic niches with fluctuating diet sources and microbial communities. However, how niche's diet and microbial composition impact mosquito performance (i.e., traits that maximize mosquito fitness) are not well understood. In this study, we focused on the Asian tiger mosquito, Aedes albopictus, one of the most invasive species in the world and a competent vector for human pathogens. To remove any external microbes, Ae. albopictus eggs were surface-sterilized then hatching larvae were exposed to a gradient of bacterial inoculum (i.e., initial microbial load) and diet concentrations while their impact on mosquito performance traits during juvenile development was measured.

Results: Our results showed that Ae. albopictus larvae develop faster and give larger adults when exposed to microbiota in rearing water. However, mosquito performance, up to the adult stage, depends on both bacterial inoculum size and diet concentration in the aquatic habitat. Upon low inoculum size, larvae survived better if the diet was in sufficient amounts whereas a higher inoculum size was associated with optimal larvae survival only in the presence of the lower amount of diet. Inoculum size, and to a lesser extent diet concentration, shaped bacterial community structure and composition of larval-rearing water allowing the identification of bacterial taxa for which their abundance in larvae-rearing water correlated with niche parameters and/or larval traits.

Conclusions: Our work demonstrates that both diet concentration and bacterial inoculum size impact mosquito performance possibly by shaping bacterial community structure in the larval habitat, which accounts for a large part of the juvenile's microbiota. Host-microbe interactions influence several mosquito life-history traits, and our work reveals that niche parameters such as inoculum size and diet concentration could have numerous implications on the microbiota assembly and host evolutionary trajectory. This underlies that host-microbe-environment interactions are an important yet overlooked factor of mosquito adaptation to its local environment, with potential future implications for vector control and vector ecology. Video Abstract.

幼虫栖息地中饮食浓度和细菌接种量的变化决定了亚洲虎蚊白纹伊蚊的表现。
背景:生态位表现出独特的环境和生物标志,如非生物条件、养分有效性和可能影响生物体生态的营养相互作用。雌蚊产卵于饮食来源和微生物群落波动的水生生态位。然而,生态位的饮食和微生物组成如何影响蚊子的表现(即最大化蚊子适应性的特征)尚不清楚。本研究以亚洲虎蚊白纹伊蚊为研究对象,白纹伊蚊是世界上最具入侵性的物种之一,也是人类病原体的主要传播媒介。为了去除任何外部微生物,Ae。对白纹伊蚊卵进行表面消毒,然后将孵化的白纹伊蚊幼虫暴露于不同的细菌接种量(即初始微生物负荷)和饮食浓度梯度中,观察其对幼蚊发育过程中性能性状的影响。结果:本研究结果表明,Ae;当白纹伊蚊幼虫暴露于饲养水中的微生物群时,其发育速度更快,成虫体型更大。然而,蚊子的表现,直到成虫阶段,取决于细菌接种量的大小和水中栖息地的饮食浓度。在低接种量的情况下,如果饲料量足够,幼虫的存活率更好,而较高的接种量只有在饲料量较低的情况下才与最佳的幼虫存活率相关。接种量的大小,以及在较小程度上的饲料浓度,塑造了幼虫养殖水中的细菌群落结构和组成,从而确定了细菌类群,它们在幼虫养殖水中的丰度与生态位参数和/或幼虫性状相关。结论:我们的工作表明,饮食浓度和细菌接种量可能通过塑造幼虫栖息地的细菌群落结构来影响蚊子的表现,这占了幼蚊微生物群的很大一部分。宿主-微生物相互作用影响蚊子的几个生活史特征,我们的工作表明,生态位参数如接种量和饮食浓度可能对微生物群聚集和宿主进化轨迹有许多影响。这表明宿主-微生物-环境相互作用是蚊子适应当地环境的一个重要但被忽视的因素,对病媒控制和病媒生态学具有潜在的未来影响。视频摘要。
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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