Porcine nasal organoids to model interactions between the swine nasal microbiota and the host.

IF 13.8 1区生物学 Q1 MICROBIOLOGY

Microbiome Pub Date : 2025-05-22 DOI:10.1186/s40168-025-02088-9

Laura Bonillo-Lopez, Noelia Carmona-Vicente, Ferran Tarrés-Freixas, Karl Kochanowski, Jorge Martínez, Mònica Perez, Marina Sibila, Florencia Correa-Fiz, Virginia Aragon

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Abstract

Background: Interactions between the nasal epithelium, commensal nasal microbiota, and respiratory pathogens play a key role in respiratory infections. Currently, there is a lack of experimental models to study such interactions under defined in vitro conditions. Here, we developed a porcine nasal organoid (PNO) system from nasal tissue of pigs as well as from cytological brushes.

Results: PNOs exhibited similar structure and cell types to the nasal mucosa, as evaluated by immunostaining. PNOs were inoculated with porcine commensal strains of Moraxella pluranimalium, Rothia nasimurium, and the pathobiont Glaesserella parasuis for examining host-commensal-pathogen interactions. All strains adhered to the PNOs, although at different levels. M. pluranimalium and G. parasuis strains stimulated the production of proinflammatory cytokines, whereas R. nasimurium induced the production of IFNγ and diminished the proinflammatory effect of the other strains.

Conclusions: Overall, PNOs mimic the in vivo nasal mucosa and can be useful to perform host-microbe interaction studies. Video Abstract.

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猪鼻类器官模拟猪鼻微生物群与宿主之间的相互作用。

背景：鼻上皮、共生鼻微生物群和呼吸道病原体之间的相互作用在呼吸道感染中起关键作用。目前，缺乏在体外条件下研究这种相互作用的实验模型。在这里，我们从猪的鼻腔组织和细胞学刷子中开发了猪鼻类器官（PNO）系统。结果：经免疫染色观察，PNOs具有与鼻黏膜相似的结构和细胞类型。将猪多动物莫拉氏菌（Moraxella pluranimium）、罗氏菌（Rothia nasimurium）和副猪绿杆菌（Glaesserella副猪）的共生菌株接种PNOs，以检测宿主-共生-病原体的相互作用。所有菌株均粘附PNOs，但程度不同。多动物分枝杆菌和副猪分枝杆菌能刺激促炎细胞因子的产生，而nasimurium能诱导IFNγ的产生，抑制其他菌株的促炎作用。结论：总的来说，PNOs模拟体内鼻黏膜，可用于进行宿主-微生物相互作用的研究。视频摘要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiome MICROBIOLOGY-

CiteScore

21.90

自引率

2.60%

发文量

198

审稿时长

4 weeks

期刊介绍： Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.