{"title":"Knockdown of the CALHM1 Gene Alleviates Allodynia in Rats With Trigeminal Neuralgia.","authors":"Xiaolei Zhu, Zhang Yi, Xuelian Li, Kaiming Duan, Dong Huang, Xuebin Yan","doi":"10.1111/joor.13980","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The sudden onset of trigeminal neuralgia is very similar to epilepsy. CALHM1 is associated with the development of temporal lobe epilepsy. Therefore, does CALHM1 play an important role in the pathogenesis of trigeminal neuralgia? In this study, we explored the effects of pain behaviour in rats with trigeminal neuralgia by knocking down the Calcium homeostasis modulator 1 gene.</p><p><strong>Methods: </strong>Rats were randomly divided into the Control group, Sham group and dION-CCI group. Animal models of trigeminal neuralgia were constructed by ligating the distal infraorbital nerve of rats with a chromic gut wire; mechanical pain thresholds and spontaneous pain behaviour were measured in rats. The trigeminal spinal subnucleus caudalis was taken on day 15 postoperatively, and expression of CALHM1 was detected by qRT-PCR and western blot. The shCALHM1 group used adeno-associated viral transfection to downregulate CALHM1 expression and detected the effect of CALHM1 knockdown by immunohistochemistry. Then the dION-CCI model was established after 3 weeks. The alterations in CALHM1 expression were identified using western blot, and the modifications in pain response in rats were observed.</p><p><strong>Results: </strong>The mRNA and protein expression of CALHM1 were significantly increased in the dION-CCI group. Knockdown of the CALHM1 gene attenuated the nociceptive allodynia in rats with trigeminal neuralgia.</p><p><strong>Conclusion: </strong>This experiment demonstrated that the occurrence of trigeminal neuralgia may be associated with increased expression of CALHM1.</p>","PeriodicalId":16605,"journal":{"name":"Journal of oral rehabilitation","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral rehabilitation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/joor.13980","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The sudden onset of trigeminal neuralgia is very similar to epilepsy. CALHM1 is associated with the development of temporal lobe epilepsy. Therefore, does CALHM1 play an important role in the pathogenesis of trigeminal neuralgia? In this study, we explored the effects of pain behaviour in rats with trigeminal neuralgia by knocking down the Calcium homeostasis modulator 1 gene.
Methods: Rats were randomly divided into the Control group, Sham group and dION-CCI group. Animal models of trigeminal neuralgia were constructed by ligating the distal infraorbital nerve of rats with a chromic gut wire; mechanical pain thresholds and spontaneous pain behaviour were measured in rats. The trigeminal spinal subnucleus caudalis was taken on day 15 postoperatively, and expression of CALHM1 was detected by qRT-PCR and western blot. The shCALHM1 group used adeno-associated viral transfection to downregulate CALHM1 expression and detected the effect of CALHM1 knockdown by immunohistochemistry. Then the dION-CCI model was established after 3 weeks. The alterations in CALHM1 expression were identified using western blot, and the modifications in pain response in rats were observed.
Results: The mRNA and protein expression of CALHM1 were significantly increased in the dION-CCI group. Knockdown of the CALHM1 gene attenuated the nociceptive allodynia in rats with trigeminal neuralgia.
Conclusion: This experiment demonstrated that the occurrence of trigeminal neuralgia may be associated with increased expression of CALHM1.
期刊介绍：
Journal of Oral Rehabilitation aims to be the most prestigious journal of dental research within all aspects of oral rehabilitation and applied oral physiology. It covers all diagnostic and clinical management aspects necessary to re-establish a subjective and objective harmonious oral function.
Oral rehabilitation may become necessary as a result of developmental or acquired disturbances in the orofacial region, orofacial traumas, or a variety of dental and oral diseases (primarily dental caries and periodontal diseases) and orofacial pain conditions. As such, oral rehabilitation in the twenty-first century is a matter of skilful diagnosis and minimal, appropriate intervention, the nature of which is intimately linked to a profound knowledge of oral physiology, oral biology, and dental and oral pathology.
The scientific content of the journal therefore strives to reflect the best of evidence-based clinical dentistry. Modern clinical management should be based on solid scientific evidence gathered about diagnostic procedures and the properties and efficacy of the chosen intervention (e.g. material science, biological, toxicological, pharmacological or psychological aspects). The content of the journal also reflects documentation of the possible side-effects of rehabilitation, and includes prognostic perspectives of the treatment modalities chosen.
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