Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING

Regenerative medicine Pub Date : 2025-04-01 Epub Date: 2025-05-23 DOI:10.1080/17460751.2025.2507480

Kevin Silva Muller, Felipe Cantore Tiburcio, Rinaldo Jose Ortiz, Rui Seabra Ferreira Junior, Benedito Barraviera, José de Anchieta de Castro E Horta, Selma Maria Michelin Matheus

{"title":"Muscle atrophy and fibrosis are attenuated after experimental nerve repair associated with heterologous fibrin biopolymer.","authors":"Kevin Silva Muller, Felipe Cantore Tiburcio, Rinaldo Jose Ortiz, Rui Seabra Ferreira Junior, Benedito Barraviera, José de Anchieta de Castro E Horta, Selma Maria Michelin Matheus","doi":"10.1080/17460751.2025.2507480","DOIUrl":null,"url":null,"abstract":"Background: Neurotmesis leads to neuromuscular junction (NMJ) degeneration, muscle atrophy, and functional loss. While neurorrhaphy is standard, motor recovery is often incomplete. Heterologous fibrin biopolymer (HFB) shows potential as an adjunct, hence, we investigate HFB's late regenerative effects.Material/methods: Twenty adult male Wistar rats (CEUA-FMB 1402/2021) were divided into Control (C), Denervated (D), Neurorrhaphy (N), and Neurorrhaphy + HFB (NB) groups. After 120 days, nerves and muscles were analyzed.Results: NB (1355 ± 170.4) showed more intact axons than C (927 ± 170.4, p = .0026) and N (774 ± 158.2, p = .0002). NMJ morphology in NB was closer to C than N, with increased nAChR alpha-1 (NB vs. N p = .0428; NB vs C p = .0084) and Rapsyn (NB vs. N p = .0130; NB vs C p = .0053) expression. Muscle integrity in NB resembled C, exhibiting less atrophy (area: C vs. N p = .0002; NB vs. N p = .0117; perimeter: C vs. N p = .0002; NB vs. N p = .0114; central nuclei: C vs. N p = .0009; NB vs. N p = .0110) and fibrosis (C vs. N p = .0061; N vs. NB p = .0326) compared to N.Conclusion: HFB associated with neurorrhaphy enhanced muscle and nerve regeneration, attenuating muscle atrophy and fibrosis.","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"133-146"},"PeriodicalIF":2.4000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118392/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regenerative medicine","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/17460751.2025.2507480","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/23 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Neurotmesis leads to neuromuscular junction (NMJ) degeneration, muscle atrophy, and functional loss. While neurorrhaphy is standard, motor recovery is often incomplete. Heterologous fibrin biopolymer (HFB) shows potential as an adjunct, hence, we investigate HFB's late regenerative effects.

Material/methods: Twenty adult male Wistar rats (CEUA-FMB 1402/2021) were divided into Control (C), Denervated (D), Neurorrhaphy (N), and Neurorrhaphy + HFB (NB) groups. After 120 days, nerves and muscles were analyzed.

Results: NB (1355 ± 170.4) showed more intact axons than C (927 ± 170.4, p = .0026) and N (774 ± 158.2, p = .0002). NMJ morphology in NB was closer to C than N, with increased nAChR alpha-1 (NB vs. N p = .0428; NB vs C p = .0084) and Rapsyn (NB vs. N p = .0130; NB vs C p = .0053) expression. Muscle integrity in NB resembled C, exhibiting less atrophy (area: C vs. N p = .0002; NB vs. N p = .0117; perimeter: C vs. N p = .0002; NB vs. N p = .0114; central nuclei: C vs. N p = .0009; NB vs. N p = .0110) and fibrosis (C vs. N p = .0061; N vs. NB p = .0326) compared to N.

Conclusion: HFB associated with neurorrhaphy enhanced muscle and nerve regeneration, attenuating muscle atrophy and fibrosis.

查看原文本刊更多论文

异种纤维蛋白生物聚合物可减轻实验性神经修复后的肌肉萎缩和纤维化。

背景：神经衰弱导致神经肌肉连接处（NMJ）变性、肌肉萎缩和功能丧失。虽然神经吻合是标准的，但运动恢复往往不完全。异种纤维蛋白生物聚合物（HFB）显示出作为辅助材料的潜力，因此，我们研究了HFB的晚期再生作用。材料/方法：20只成年雄性Wistar大鼠（CEUA-FMB 1402/2021）分为对照组(C)、去神经组(D)、神经吻合组(N)和神经吻合+ HFB （NB）组。120天后，对神经和肌肉进行分析。结果：NB（1355±170.4）比C（927±170.4,p = 0.0026）和N（774±158.2,p = 0.0002）显示更多完整的轴突。NB组NMJ形态更接近于C组，nAChR α -1升高(NB vs. N p = 0.0428；NB vs C p = .0084)和Rapsyn (NB vs. N p = .0130；NB vs C p = .0053)表达。NB的肌肉完整性与C相似，表现出较小的萎缩(面积：C vs. N p = 0.0002；NB vs. N p = 0.0117；周长：C vs. N p = 0.0002；NB vs. N p = 0.0114；中央核：C vs. N p = 0.0009；NB vs. N p = 0.0110)和纤维化(C vs. N p = 0.0061；结论：HFB与神经缝合相关，增强肌肉和神经再生，减轻肌肉萎缩和纤维化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.