Attenuated notch signaling decreases T-cell factor-1+ Treg subsets in lung adenocarcinoma, assisting early patient screening

IF 3.3 4区医学 Q3 IMMUNOLOGY

Immunology letters Pub Date : 2025-05-20 DOI:10.1016/j.imlet.2025.107034

Ayibaota Bahabayi , Mohan Zheng , Ainizati Hasimu , Rui Kang , Qi Li , Ziqi Xiong , Zhao Guan , Zhonghui Zhang , Tianci Liu , Xingyue Zeng , Chen Liu

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引用次数: 0

Abstract

Objective

This study aimed to investigate the role of T-cell factor-1 (TCF1) in early-stage lung adenocarcinoma (LUAD) patients and explore its clinical significance for diagnosing early LUAD.

Methods

Blood samples were collected from 34 stage IA LUAD patients and 31 healthy controls. Flow cytometry was used to analyze the levels of TCF1 in circulating T cell subpopulations. Functional characteristics of TCF1-related cells were investigated by staining with CD62L and Ki-67. Changes in TCF1-related proportions in T cell subsets of early LUAD patients were analyzed. The role of Notch signaling was clarified by adding the Notch signal activator Jagged1 (JAG1). Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic value of TCF1-related T cell subsets for screening early LUAD.

Results

The expression level of TCF1 in follicular regulatory T(Tfr) and regulatory T(Treg) cells was decreased in early LUAD patients, and TCF1+CD62L+ follicular helper (Tfh) cells were also decreased. TCF1+CD62L+ cells in both Treg and Tfr were decreased in early LUAD patients. Decreased TCF1 in Treg and Tfr recovered in early LUAD after adding JAG1. TCF1-related indicators showed good auxiliary diagnostic significance for early LUAD. TCF1+, TCF1+CD62L+, and TCF1-CD62L+ percentages in Treg and Tfr cells were with areas under the curve (AUCs) between 0.827 and 0.897 to distinguish early LUAD from healthy individuals.

Conclusions

Downregulation of Notch signaling contributes to the decrease in TCF1+ Treg subsets in LUAD patients, which is of significant value for screening early-stage lung adenocarcinoma.

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减弱的Notch信号减少了肺腺癌中的t细胞因子-1+ Treg亚群，有助于早期患者筛查。

目的：本研究旨在探讨t细胞因子-1 （TCF1）在早期肺腺癌（LUAD）患者中的作用，探讨其对早期LUAD诊断的临床意义。方法：采集34例IA期LUAD患者和31例健康对照者的血液标本。流式细胞术用于分析循环T细胞亚群中TCF1的水平。通过CD62L和Ki-67染色研究tcf1相关细胞的功能特征。分析早期LUAD患者T细胞亚群中tcf1相关比例的变化。通过加入Notch信号激活因子Jagged1 （JAG1）阐明Notch信号的作用。采用受试者工作特征（ROC）曲线评价tcf1相关T细胞亚群对筛查早期LUAD的诊断价值。结果：早期LUAD患者滤泡调节性T细胞（Tfr）和调节性T细胞（Treg）中TCF1表达水平降低，TCF1+CD62L+滤泡辅助细胞（Tfh）表达水平也降低。早期LUAD患者Treg和Tfr中TCF1+CD62L+细胞均降低。添加JAG1后，Treg中TCF1降低，LUAD早期Tfr恢复。tcf1相关指标对早期LUAD有很好的辅助诊断意义。Treg和Tfr细胞中TCF1+、TCF1+CD62L+和TCF1-CD62L+百分比曲线下面积（aus）在0.827 ~ 0.897之间，可以区分早期LUAD与健康个体。结论：Notch信号下调导致LUAD患者TCF1+ Treg亚群减少，对早期肺腺癌筛查具有重要价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunology letters 医学-免疫学

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

44 days

期刊介绍： Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.