Hsa_circ_0006837 suppresses gastric cancer cell proliferation, migration, and invasion via the modulation of miR-424-5p.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-05-22 DOI:10.1186/s41065-025-00449-w

Yanxin He, Yeyu Sun, Yinglan Zheng, Yanfang Jiang, Na Li, Wenjie Zhao, Wanhua Ren

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引用次数: 0

Abstract

Background: The mechanism by which circRERE (hsa_circ_0006837) modulates the malignant progression of gastric cancer was investigated to identify a novel biomarker and therapeutic target for this disease.

Methods: Hsa_circ_0006837 expression in GC tissues and cells was detected by RT-qPCR. Several data analysis methods were used to evaluate the significance of dysregulated hsa_circ_0006837 in GC. The patients were followed up for five years, and survival analysis was conducted using Kaplan-Meier curves. Cox regression was subsequently performed to analyze the risk factors for prognosis. The malignant behaviors of the cells were detected by the CCK-8 and Transwell assays. The relationship between hsa_circ_0006837 and miR-424-5p was assessed by conducting Spearman correlation analysis and verified by dual-luciferase reporter assay.

Results: Hsa_circ_0006837 expression decreased in patients with GC, indicating a poorer patient prognosis. In GC cells, hsa_circ_0006837 overexpression suppressed malignant behaviors. Mechanistically, miR-424-5p was identified as a target of hsa_circ_0006837. The overexpression of miR-424-5p partially counteracted the suppressive effects of upregulated hsa_circ_0006837 on the malignant behaviors of GC cells. FBXO21 was identified as a downstream gene of the hsa_circ_0006837/miR-424-5p axis.

Conclusions: To summarize, hsa_circ_0006837 is a biomarker for the prognosis of GC. Mechanistically, hsa_circ_0006837 overexpression can modulate the malignant behaviors of GC cells through miR-424-5p.

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Hsa_circ_0006837通过调控miR-424-5p抑制胃癌细胞的增殖、迁移和侵袭。

背景：研究circRERE （hsa_circ_0006837）调控胃癌恶性进展的机制，以确定胃癌的新生物标志物和治疗靶点。方法：采用RT-qPCR检测Hsa_circ_0006837在GC组织和细胞中的表达。采用多种数据分析方法评价hsa_circ_0006837基因异常在GC中的意义。随访5年，采用Kaplan-Meier曲线进行生存分析。随后采用Cox回归分析影响预后的危险因素。CCK-8和Transwell检测细胞的恶性行为。通过Spearman相关分析评估hsa_circ_0006837与miR-424-5p的关系，并通过双荧光素酶报告基因试验进行验证。结果：Hsa_circ_0006837在胃癌患者中表达降低，提示预后较差。在GC细胞中，hsa_circ_0006837过表达抑制恶性行为。机制上，miR-424-5p被确定为hsa_circ_0006837的靶标。miR-424-5p的过表达部分抵消了上调的hsa_circ_0006837对GC细胞恶性行为的抑制作用。FBXO21被鉴定为hsa_circ_0006837/miR-424-5p轴的下游基因。结论：综上所述，hsa_circ_0006837是胃癌预后的生物标志物。机制上，hsa_circ_0006837过表达可通过miR-424-5p调控GC细胞的恶性行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.