Synthesis of 99mTc-labeled polyaspartic acid/silica nanoassembly as a potential probe for bone imaging

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

BMC Chemistry Pub Date : 2025-05-24 DOI:10.1186/s13065-025-01508-z

Noha A. Bayoumi, Marwa E. Sayyed, Wael M. Darwish

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引用次数: 0

Abstract

Purpose

Due to the efficient bone targeting of mesoporous silica nanoparticles (MSNs) and polyaspartic acid (PASP), ^99mTc- labeled polyaspartic acid coated mesoporous silica nanoparticles (PASP-mSiO₂-DTPA-^99mTc) are proposed as a potential probe for bone imaging.

Methods

Polyaspartic acid-conjugated silica nanoparticles (PASP-mSiO₂) were synthesized using aqueous carbodiimide chemistry and characterized by ATR-FTR, FE-SEM, EDX, TEM, TGA and XRD. Radiolabeling of the produced nanoassembly with ^99mTc was carried out via a simple DTPA chelation procedure. Aqueous dispersion of the radiolabeled nanoparticles was intravenously injected into normal mice and the bone targeting efficiency was evaluated.

Results

The PASP-mSiO₂ nanoassembly was efficiently synthesized and radiolabeled with ^99mTc with a high radiochemical yield (92 ± 0.5%) and sufficient in vitro stability in PBS and FBS for up to 24 h. In vivo biodistribution studies revealed a significant enhancement of radioactivity bone uptake after intravenous injection of PASP-mSiO₂-DTPA-^99mTc compared to radiolabeled uncoated MSNs (mSiO₂-DTPA-^99mTc), (13 ± 0.6% IA/gram and 5.4 ± 0.4, respectively).

Conclusion

PASP endowed MSNs with enhanced biocompatibility and highly selective bone targeting. Therefore, the proposed PASP-mSiO₂-DTPA-^99mTc nanoassembly has immense potential in the field of bone- imaging via single photon emitting computed tomography (SPECT).

Graphical Abstract

查看原文本刊更多论文

99mtc标记聚天冬氨酸/二氧化硅纳米复合物的合成及其在骨成像中的应用

由于介孔二氧化硅纳米颗粒（MSNs）和聚天冬氨酸（PASP）具有高效的骨靶向性，99mTc标记的聚天冬氨酸包被介孔二氧化硅纳米颗粒（PASP- msio2 - dtpa -99mTc）被认为是一种潜在的骨成像探针。方法采用水相碳二亚胺化学方法合成聚天冬氨酸共轭二氧化硅纳米粒子（PASP-mSiO2），并采用ATR-FTR、FE-SEM、EDX、TEM、TGA和XRD等手段对其进行表征。通过简单的DTPA螯合程序，用99mTc对生成的纳米组件进行放射性标记。将放射性标记纳米颗粒的水分散体静脉注射到正常小鼠体内，并评估其骨靶向效率。结果PASP-mSiO2纳米组件被有效合成，并被99mTc放射性标记，具有较高的放射化学产率（92±0.5%），并且在PBS和FBS中具有长达24小时的体外稳定性。体内生物分布研究显示，静脉注射PASP-mSiO2- dtpa -99mTc后，与放射性标记的未包被MSNs （mSiO2-DTPA-99mTc）相比，放射性骨摄取显著增强（分别为13±0.6% IA/g和5.4±0.4）。结论pasp可增强骨髓间充质干细胞的生物相容性和高选择性骨靶向性。因此，所提出的PASP-mSiO2-DTPA-99mTc纳米组件在单光子发射计算机断层扫描（SPECT）骨成像领域具有巨大的潜力。图形抽象。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Chemistry Chemistry-General Chemistry

CiteScore

5.30

自引率

2.20%

发文量

审稿时长

27 weeks

期刊介绍： BMC Chemistry, formerly known as Chemistry Central Journal, is now part of the BMC series journals family. Chemistry Central Journal has served the chemistry community as a trusted open access resource for more than 10 years – and we are delighted to announce the next step on its journey. In January 2019 the journal has been renamed BMC Chemistry and now strengthens the BMC series footprint in the physical sciences by publishing quality articles and by pushing the boundaries of open chemistry.