Hydrodynamic cavitation as a new approach to enhance the enzymatic hydrolysis of sugarcane bagasse

IF 4.8 2区 工程技术 Q1 MATERIALS SCIENCE, PAPER & WOOD
C. A. Prado, P. A. F. H. P. Fernandes, M. M. Cruz-Santos, M. L. S. Cunha, R. Terán-Hilares, P. R. F. Marcelino, S. S. da Silva, J. C. Santos
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Abstract

A pretreatment step is essential to improve the enzymatic digestibility of biomass, and in recent years, hydrodynamic cavitation (HC) has emerged as a promising alternative. However, while HC has been primarily studied for pretreatment, its application to directly assist the enzymatic hydrolysis process remains unexplored. In this work, a novel approach was introduced, and the effect of HC on the enzymatic hydrolysis of sugarcane bagasse was evaluated by performing a face centered central composite design with enzyme loading and temperature as variables. The analysis of the design results and the process optimization were aided by Design-Expert and Statistica software. Additionally, a sequential strategy involving HC-assisted enzymatic hydrolysis was implemented through four cycles of fed-batch processing. Under optimized conditions (enzyme loading of 18 FPU/g and temperature of 50 °C), glucan and xylan hydrolysis yields of 91% and 77%, respectively, were achieved after 12 h. These yields surpassed those obtained in control experiments performed with ultrasound-assisted hydrolysis. In fed-batch process, the obtained sugar concentrations were increased 5–6 times in the hydrolysate compared to the values obtained in simple batch mode. The results demonstrate HC as an innovative and effective alternative for enhancing enzymatic hydrolysis.

Graphical abstract

Abstract Image

流体动力空化是促进蔗渣酶解的新途径
预处理步骤是提高生物质酶消化率所必需的,近年来,流体动力空化(HC)已成为一种有前途的替代方法。然而,虽然HC主要用于预处理研究,但其直接辅助酶解过程的应用仍未探索。本文介绍了一种新的方法,通过以酶载量和温度为变量的面中心中心复合设计,评价了HC对甘蔗渣酶解的影响。利用design - expert和Statistica软件对设计结果进行分析和工艺优化。此外,涉及hc辅助酶解的顺序策略通过四个进料批处理循环实施。在最佳条件下(酶载量为18 FPU/g,温度为50°C), 12 h后葡聚糖和木聚糖的水解率分别为91%和77%,超过了超声辅助水解的对照实验。在间歇进料过程中,获得的糖浓度在水解液中增加了5-6倍,与在简单间歇模式中获得的值相比。结果表明,HC是一种创新和有效的替代增强酶水解。图形抽象
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来源期刊
Cellulose
Cellulose 工程技术-材料科学:纺织
CiteScore
10.10
自引率
10.50%
发文量
580
审稿时长
3-8 weeks
期刊介绍: Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.
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