C. A. Prado, P. A. F. H. P. Fernandes, M. M. Cruz-Santos, M. L. S. Cunha, R. Terán-Hilares, P. R. F. Marcelino, S. S. da Silva, J. C. Santos
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引用次数: 0
Abstract
A pretreatment step is essential to improve the enzymatic digestibility of biomass, and in recent years, hydrodynamic cavitation (HC) has emerged as a promising alternative. However, while HC has been primarily studied for pretreatment, its application to directly assist the enzymatic hydrolysis process remains unexplored. In this work, a novel approach was introduced, and the effect of HC on the enzymatic hydrolysis of sugarcane bagasse was evaluated by performing a face centered central composite design with enzyme loading and temperature as variables. The analysis of the design results and the process optimization were aided by Design-Expert and Statistica software. Additionally, a sequential strategy involving HC-assisted enzymatic hydrolysis was implemented through four cycles of fed-batch processing. Under optimized conditions (enzyme loading of 18 FPU/g and temperature of 50 °C), glucan and xylan hydrolysis yields of 91% and 77%, respectively, were achieved after 12 h. These yields surpassed those obtained in control experiments performed with ultrasound-assisted hydrolysis. In fed-batch process, the obtained sugar concentrations were increased 5–6 times in the hydrolysate compared to the values obtained in simple batch mode. The results demonstrate HC as an innovative and effective alternative for enhancing enzymatic hydrolysis.
期刊介绍:
Cellulose is an international journal devoted to the dissemination of research and scientific and technological progress in the field of cellulose and related naturally occurring polymers. The journal is concerned with the pure and applied science of cellulose and related materials, and also with the development of relevant new technologies. This includes the chemistry, biochemistry, physics and materials science of cellulose and its sources, including wood and other biomass resources, and their derivatives. Coverage extends to the conversion of these polymers and resources into manufactured goods, such as pulp, paper, textiles, and manufactured as well natural fibers, and to the chemistry of materials used in their processing. Cellulose publishes review articles, research papers, and technical notes.