The impact of metagenomic analysis on the discovery of novel endolysins

IF 3.9 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2025-05-24 DOI:10.1007/s00253-025-13513-2

Nikolaos Georgakis, Georgios E. Premetis, Panagiota Pantiora, Christina Varotsou, Charoutioun S. Bodourian, Nikolaos E. Labrou

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引用次数: 0

Abstract

Metagenomics has revolutionized enzyme discovery by enabling the study of genetic material directly from environmental samples, bypassing the need for microbial cultivation. This approach is particularly effective for identifying novel endolysins, phage-derived enzymes with antibacterial properties suited for therapeutic and industrial applications. Diverse ecosystems, such as biofilms, human microbiome, hot springs, and geothermal areas, serve as rich reservoirs for endolysins with traits like thermostability, broad-spectrum activity, specificity and resistance to harsh conditions. Functional metagenomics, complemented by bioinformatics, enables the discovery and annotation of previously uncharacterized endolysins. Examples of endolysins discovered from metagenomics analysis are discussed. Despite the challenges of analyzing complex microbial ecosystems and isolating target genes, metagenomics holds immense potential for uncovering innovative endolysins, paving the way for developing new biotechnological applications.

• Endolysins offer antibacterial potential for therapeutic and industrial use.

• Metagenomics enables discovery of novel endolysins from diverse ecosystems.

• Advances in tools and methods have accelerated novel endolysins discovery.

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宏基因组分析对发现新型内溶素的影响

宏基因组学通过直接从环境样本中研究遗传物质，绕过微生物培养的需要，彻底改变了酶的发现。这种方法对鉴定新型内溶素特别有效，内溶素是噬菌体衍生的酶，具有抗菌特性，适合于治疗和工业应用。不同的生态系统，如生物膜、人类微生物群、温泉和地热区，是内溶素的丰富储存库，具有热稳定性、广谱活性、特异性和对恶劣条件的抗性等特征。功能宏基因组学，辅以生物信息学，使以前未表征的内溶酶的发现和注释。讨论了从宏基因组学分析中发现的内溶素的例子。尽管在分析复杂的微生物生态系统和分离目标基因方面存在挑战，但宏基因组学在发现创新的内溶素方面具有巨大的潜力，为开发新的生物技术应用铺平了道路。•内溶素为治疗和工业用途提供抗菌潜力。•宏基因组学可以从不同的生态系统中发现新的内溶素。•工具和方法的进步加速了新型内溶素的发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.