Investigation the forensic efficacy and genetic trait of a Chinese ethnic group by 57 autosomal InDel loci

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-05-14 DOI:10.1016/j.genrep.2025.102253

Xiaolian Wu , Qinglin Liang , Qinglin Liu , Lisiteng Luo , Meiming Cai , Yanfang Liu , Meisen Shi , Bofeng Zhu

{"title":"Investigation the forensic efficacy and genetic trait of a Chinese ethnic group by 57 autosomal InDel loci","authors":"Xiaolian Wu , Qinglin Liang , Qinglin Liu , Lisiteng Luo , Meiming Cai , Yanfang Liu , Meisen Shi , Bofeng Zhu","doi":"10.1016/j.genrep.2025.102253","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>The 60-Plex InDel panel has recently been developed for forensic individual identification, and has demonstrated high sensitivity, reproducibility, and stability through various validation tests. However, its effectiveness still requires further validation across diverse populations to expand the population genetic database.</div></div><div><h3>Materials and methods</h3><div>In this study, we applied the 60-Plex InDel panel to genotype 190 unrelated healthy individuals from Chinese Manchu group. Through a series of genetic analyses, we evaluated the forensic efficacy of this panel in Chinese Manchu group.</div></div><div><h3>Results</h3><div>The 57 autosomal InDel loci showed high polymorphisms in Chinese Manchu group. The cumulative probability of matching and cumulative power of exclusion values of this panel were 4.4465 × 10<sup>–24</sup> and 0.9999808285 in Chinese Manchu group, respectively. Full sibling identification accuracy reached 82.90 % in Chinese Manchu group with the likelihood ratio limit of 100. Population genetic analyses, including <em>D</em><sub><em>A</em></sub> distances, <em>F</em><sub><em>ST</em></sub> values, phylogenetic tree construction, principal component analysis and STRUCTURE analysis, revealed that the Chinese Manchu group shared genetic proximity with the East Asian populations. The biogeographic origins of three continental populations (African, East Asian, and European) were inferred with high accuracy using two classification models (Random Forest and Support Vector Machine).</div></div><div><h3>Conclusions</h3><div>The 60 Plex-InDel panel is powerful for individual identification and paternity testing in Chinese Manchu group. It also has potential in inferring the biogeographic origins of individuals from different continents. The Chinese Manchu group is genetically related to East Asian populations, particularly to the Chinese Beijing Han population.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"40 ","pages":"Article 102253"},"PeriodicalIF":0.9000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425001268","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

The 60-Plex InDel panel has recently been developed for forensic individual identification, and has demonstrated high sensitivity, reproducibility, and stability through various validation tests. However, its effectiveness still requires further validation across diverse populations to expand the population genetic database.

Materials and methods

In this study, we applied the 60-Plex InDel panel to genotype 190 unrelated healthy individuals from Chinese Manchu group. Through a series of genetic analyses, we evaluated the forensic efficacy of this panel in Chinese Manchu group.

Results

The 57 autosomal InDel loci showed high polymorphisms in Chinese Manchu group. The cumulative probability of matching and cumulative power of exclusion values of this panel were 4.4465 × 10^–24 and 0.9999808285 in Chinese Manchu group, respectively. Full sibling identification accuracy reached 82.90 % in Chinese Manchu group with the likelihood ratio limit of 100. Population genetic analyses, including D_A distances, F_ST values, phylogenetic tree construction, principal component analysis and STRUCTURE analysis, revealed that the Chinese Manchu group shared genetic proximity with the East Asian populations. The biogeographic origins of three continental populations (African, East Asian, and European) were inferred with high accuracy using two classification models (Random Forest and Support Vector Machine).

Conclusions

The 60 Plex-InDel panel is powerful for individual identification and paternity testing in Chinese Manchu group. It also has potential in inferring the biogeographic origins of individuals from different continents. The Chinese Manchu group is genetically related to East Asian populations, particularly to the Chinese Beijing Han population.

查看原文本刊更多论文

57个常染色体InDel位点对某华人群体法医学效力及遗传性状的研究

60-Plex InDel面板最近被开发用于法医个体识别，并通过各种验证测试显示出高灵敏度、可重复性和稳定性。然而，其有效性仍需要在不同种群中进一步验证，以扩大种群遗传数据库。材料与方法本研究采用60-Plex InDel面板对中国满族190例无亲缘关系的健康个体进行基因型分析。通过一系列的遗传分析，我们评估了该小组在中国满族群体中的法医功效。结果57个常染色体InDel位点在满族人群中表现出较高的多态性。在中国满族人群中，该面板的累积匹配概率为4.4465 × 10-24，累积排除力为0.9999808285。满族全同胞识别正确率达82.90%，似然比限为100。群体遗传分析（包括DA距离、FST值、系统发育树构建、主成分分析和结构分析）表明，中国满族群体与东亚人群具有遗传接近性。利用两种分类模型（随机森林和支持向量机）对非洲、东亚和欧洲三个大陆种群的生物地理起源进行了高精度推断。结论60 Plex-InDel面板对中国满族人群的个体鉴定和亲子鉴定具有较强的应用价值。它也有可能推断来自不同大陆的个体的生物地理起源。中国满族人在基因上与东亚人口，特别是中国北京汉族人口有联系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.