High-throughput screening of 311 new psychoactive substances and metabolites in wastewater by direct injection UPLC-MS/MS

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-05-18 DOI:10.1016/j.jchromb.2025.124659

Yue Xiao , Shuai Yuan , Ruxin Luo , Ruiqin Zhu , Qiongying Zheng , Bin Di , Ping Xiang

{"title":"High-throughput screening of 311 new psychoactive substances and metabolites in wastewater by direct injection UPLC-MS/MS","authors":"Yue Xiao , Shuai Yuan , Ruxin Luo , Ruiqin Zhu , Qiongying Zheng , Bin Di , Ping Xiang","doi":"10.1016/j.jchromb.2025.124659","DOIUrl":null,"url":null,"abstract":"<div><div>The rapid global spread of new psychoactive substances (NPS) in recent years has become an issue in many countries. One emerging technology that can provide early warnings and assessments of drug situations is the monitoring of NPS in wastewater. In this study, we developed and optimized a high-throughput qualitative screening method based on direct injection that enabled the simultaneous detection of 311 NPS and metabolites (87 synthetic cannabinoids, 43 synthetic cathinones, 71 phenylethylamines, 10 tryptamines, 40 phencyclidines, 9 benzodiazepines, 38 fentanyls, and 13 piperazines) in wastewater. The run time for drug detection was only 16 min, and the method was validated to perform well in terms of selectivity, limit of detection (LOD), recovery, and matrix effect. Overall, 95.8 % of the targets had an LOD ≤10 ng/L and the recoveries ranged from 71.01 % to 119.88 %. The method was validated on 976 real samples from a city in China, and 32 substances were detected, with the highest detection rate for ketamine. This study provides a simple and direct analytical method for wastewater monitoring, which will aid in combating drug-related crime and maintaining social stability as new NPS continue to enter global markets.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1262 ","pages":"Article 124659"},"PeriodicalIF":2.8000,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1570023225002132","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

The rapid global spread of new psychoactive substances (NPS) in recent years has become an issue in many countries. One emerging technology that can provide early warnings and assessments of drug situations is the monitoring of NPS in wastewater. In this study, we developed and optimized a high-throughput qualitative screening method based on direct injection that enabled the simultaneous detection of 311 NPS and metabolites (87 synthetic cannabinoids, 43 synthetic cathinones, 71 phenylethylamines, 10 tryptamines, 40 phencyclidines, 9 benzodiazepines, 38 fentanyls, and 13 piperazines) in wastewater. The run time for drug detection was only 16 min, and the method was validated to perform well in terms of selectivity, limit of detection (LOD), recovery, and matrix effect. Overall, 95.8 % of the targets had an LOD ≤10 ng/L and the recoveries ranged from 71.01 % to 119.88 %. The method was validated on 976 real samples from a city in China, and 32 substances were detected, with the highest detection rate for ketamine. This study provides a simple and direct analytical method for wastewater monitoring, which will aid in combating drug-related crime and maintaining social stability as new NPS continue to enter global markets.

查看原文本刊更多论文

直接进样UPLC-MS/MS高通量筛选废水中311种新型精神活性物质及代谢物

近年来，新型精神活性物质（NPS）在全球迅速蔓延，已成为许多国家面临的一个问题。一项能够提供药物状况早期预警和评估的新兴技术是监测废水中的NPS。本研究建立并优化了一种基于直接注射的高通量定性筛选方法，可同时检测废水中311种NPS及其代谢物（合成大麻素87种、合成卡西酮43种、苯乙胺71种、色胺10种、苯环利定40种、苯二氮平类9种、芬太尼38种、哌嗪13种）。结果表明，该方法在选择性、检出限、回收率和基质效应等方面均具有良好的效果。总体而言，95.8%的目标物LOD≤10 ng/L，回收率在71.01% ~ 119.88%之间。对中国某市976份真实样品进行验证，共检出32种物质，其中氯胺酮的检出率最高。这项研究为废水监测提供了一种简单直接的分析方法，这将有助于打击与毒品有关的犯罪和维护社会稳定，因为新的NPS不断进入全球市场。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.