Alpha-lipoic acid supplementation in pike silverside microdiets: Nutrigenomic analysis reveals potential molecular pathways leading to enhanced feeding efficiency and larvae survival

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-05-20 DOI:10.1016/j.anifeedsci.2025.116396

Mitzi Ernestina Juárez-Gutiérrez , Frida Angélica Monroy de la Peña , María Gisela Ríos-Durán , Carlos Cristian Martínez-Chávez , Jorge Fonseca-Madrigal , Sibila Concha-Santos , Carlos Antonio Martínez-Palacios , Pamela Navarrete-Ramírez

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引用次数: 0

Abstract

Alpha-lipoic acid (ALA) is an antioxidant with an increasing interest in aquaculture feeds, since ALA can positively affect performance and counteract oxidative stress, it may be an ideal supplement. A previous study in pike silverside larvae (Chirostoma estor) showed overexpression of detrimental (stress-related) genes when larvae were fed a specific microdiet compared to those exclusively fed live food. Therefore, this study evaluated the effect of different dietary ALA supplementation levels (0, 20, 40, 80, 160 mg Kg⁻¹ diet) included on microdiets in the performance and the transcriptomic profile of 7 days post-hatched pike silverside larvae for 27 days. Dietary supplementation of alpha-lipoic acid (ALA 20 and 160 mg Kg⁻¹) halved larval mortality of pike silverside larvae and improved feeding efficiency (Food Conversion Ratio of 1.07 vs. 1.67 in the control group) in this species. Differential gene expression analysis revealed an overexpression of genes involved in the signaling pathways of mitochondrial biogenesis, reactive oxygen species response, DNA repair, and increased cellular energy, supporting survival and feeding efficiency parameters observed in pike silverside larvae. This work provides evidence of the positive effects of ALA at the lowest levels reported to date as a dietary supplement in fish.

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在梭子鱼微饲料中添加α -硫辛酸：营养基因组学分析揭示了提高摄食效率和幼虫存活率的潜在分子途径

α -硫辛酸（ALA）是一种越来越受到水产养殖饲料关注的抗氧化剂，因为ALA可以积极影响生产性能和对抗氧化应激，它可能是一种理想的补充剂。先前的一项研究表明，与只喂食活饲料的幼鱼相比，喂食特定微饲料的幼鱼会过度表达有害（应激相关）基因。因此，本研究评估了微饲料中不同ALA添加水平（0、20、40、80、160 mg Kg−1）对7日龄银腹梭子鱼幼体27 d生产性能和转录组学特征的影响。饲料中添加α -硫辛酸（ALA 20和160 mg Kg−1）可使银鳉幼虫的死亡率降低一半，并提高摄食效率（食物转化率为1.07 vs.对照组为1.67）。差异基因表达分析显示，参与线粒体生物发生、活性氧反应、DNA修复和细胞能量增加的信号通路的基因过表达，支持了银鳉幼虫的生存和摄食效率参数。这项工作提供了证据，证明ALA作为鱼类膳食补充剂的积极作用是迄今为止报道的最低水平。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.