Incidence, genetic characterization and phylogenetic analysis of Cryptosporidium species infecting domestic pigeons (Columba livia domestica) in Egypt

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-05-21 DOI:10.1016/j.vprsr.2025.101283

Fatema A. Gamal, Azza M. Abdel-Wahab, Marwa M. Khalifa, Reem M. Ramadan, Ahmed M. Nassar

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Abstract

The current investigation was performed to study the incidence, morphological characters and molecular identification of Cryptosporidium species infecting domestic pigeons in the Giza governorate, Egypt. The incidence of Cryptosporidium spp. by modified Ziehl-Neelsen stain technique was 12 % and 14 % of the examined intestinal and bursal samples respectively. Seasonal incidence showed that winter had the highest rate (15.38 % and 21.74 %) while the lowest one was in autumn (7.95 % and 8.33 %) of the intestinal and bursal samples respectively. Two forms of Cryptosporidium spp. were detected. The small oocysts had average size (4.6 ± 0.63 × 4.44 ± 0.76 μm) while the large one had average size (5.64 ± 0.39 × 5.57 ± 0.41 μm). The PCR products of Cryptosporidium spp. were analyzed using two pairs of primers. The analysis revealed that the amplicons of the 18S rRNA region had 830 bp and 760 bp. Sequencing and phylogenetic analysis of the amplified products indicated that the species under investigation were C. meleagridis and C. baileyi with accession numbers OR631411 and OR631417, respectively. To our knowledge, the identification and genetic characterization of C. meleagridis and C. baileyi within the genus Cryptosporidium infecting domestic pigeons is reported for the first time in Egypt.

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埃及家鸽隐孢子虫的发病率、遗传特征及系统发育分析

对埃及吉萨省家鸽隐孢子虫的发病率、形态特征和分子鉴定进行了调查。改良Ziehl-Neelsen染色法检测的隐孢子虫在小肠和法氏囊样品中的感染率分别为12%和14%。季节发病率以冬季最高，分别为15.38%和21.74%，秋季最低，分别为7.95%和8.33%。检测到两种隐孢子虫。小卵囊平均大小为4.6±0.63 × 4.44±0.76 μm，大卵囊平均大小为5.64±0.39 × 5.57±0.41 μm。采用两对引物对隐孢子虫的PCR产物进行分析。分析结果显示，18S rRNA区扩增子分别为830 bp和760 bp。扩增产物的测序和系统发育分析表明，该菌株为C. meleagridis和C. baileyi，分别为OR631411和OR631417。据我们所知，埃及国内首次报道了感染家鸽的隐孢子虫属C. meleagridis和C. baileyi的鉴定和遗传特征。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).