Wound healing potential of Labisia pumila extracts and relationship with its phytochemical contents through in silico assessment

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-05-05 DOI:10.1016/j.hermed.2025.101024

Atiqah Azam , Shihab Uddin Ahmad , Mohiuddin Ahmed Bhuiyan , Ajoy Kumer , Sanjit Dash , Shiplu Roy Chowdhury , Ahmad Nazrun Shuid , Isa Naina Mohamed

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引用次数: 0

Abstract

Introduction

Labisia pumila (LP) has traditionally been used for women's postnatal recovery, with research highlighting its antioxidant, anti-inflammatory, antimicrobial, and phytoestrogen properties essential for wound healing. This study explores its wound-healing potential through in vitro and in silico models.

Methods

The study focused on the proliferation and migration activities of human primary skin fibroblasts and keratinocytes treated with two common LP varieties found in Malaysia: var. pumila and var. alata. leaf and root aqueous extracts.

Results

Results showed that root extracts of both LP species were toxic to skin cells at low concentrations compared to leaf extracts. Optimal concentrations of LP leaf extracts (1.5 µg/ml for fibroblasts and 3.1 µg/ml for keratinocytes) significantly increased (P < 0.05) the proliferation of fibroblasts (194000 ± 3623 cells/cm²) and keratinocytes (275578 ± 14959 cells/cm²) compared to untreated controls. Additionally, both LP leaf extracts promoted higher healing rates (P < 0.05) in fibroblasts (14791.5 ± 1615.6 μm²/h) and keratinocytes (20438 ± 2206 μm²/h) compared to the control group. Furthermore, a docking study was conducted to assess the affinity of major phytoconstituents for binding to glycogen synthase kinase 3-β (GSK3-β), a protein involved in wound healing. Ellagic acid derivative showed the highest binding affinity (-12 kcal/mol)) against GSK3-β.

Conclusion

In conclusion, both LP var. alata and var. pumila leaf extracts demonstrated the ability to enhance the proliferation and migration of fibroblasts and keratinocytes, suggesting their potential for promoting wound healing.

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阴唇提取物的创面愈合潜力及其与植物化学成分的关系

阴唇（LP）传统上用于女性产后恢复，研究强调其抗氧化，抗炎，抗菌和植物雌激素特性对伤口愈合至关重要。本研究通过体外和硅模型探索其伤口愈合潜力。方法研究马来西亚常见的两种LP品种var. pumila和var. alata对人原代皮肤成纤维细胞和角质形成细胞的增殖和迁移活性。叶和根的水提取物。结果两种植物的根提取物在低浓度下均比叶提取物对皮肤细胞有毒性。LP叶提取物的最佳浓度（成纤维细胞为1.5µg/ml，角质形成细胞为3.1µg/ml）显著增加(P <；0.05)成纤维细胞（194000±3623个细胞/cm2）和角质形成细胞（275578±14959个细胞/cm2）的增殖。此外，两种LP叶提取物都促进了更高的愈合率(P <；0.05)，成纤维细胞（14791.5±1615.6 μm2/h）和角质形成细胞（20438±2206 μm2/h）与对照组比较。此外，一项对接研究评估了主要植物成分与糖原合成酶激酶3-β (GSK3-β)结合的亲和力，GSK3-β是一种参与伤口愈合的蛋白质。鞣花酸衍生物对GSK3-β的结合亲和力最高（-12 kcal/mol）。结论紫丁香叶提取物和紫丁香叶提取物均能促进成纤维细胞和角质形成细胞的增殖和迁移，具有促进创面愈合的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.