Identification and functional characterisation of the gibberellin-inactivating enzyme, IbCYP714A1, in sweetpotato

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-29 DOI:10.1016/j.plaphy.2025.109973

Meng Xing , Minhong He , Shulin Deng , Yi Zhang , Hongbo Zhu

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引用次数: 0

Abstract

Bioactive gibberellins (GAs) are key hormones that regulate plant growth and development, playing a central role in agronomic traits such as plant height. Cytochrome P450 genes have emerged as important regulators of GA metabolism. In rice, the ELONGATED UPPERMOST INTERNODE1 (EUI1) gene, and in Arabidopsis, ELA1 (CYP714A1) and ELA2 (CYP714A2), encode P450 monooxygenases with gibberellin inactivation functions. This study aimed to identify GA-inactivating P450 genes in sweetpotato and evaluate their functional impact on plant architecture and storage root development, which is a critical yield-related trait. In this study, we successfully identified a cytochrome P450 gene, IbCYP714A1, from the sweetpotato variety Jishu26. The IbCYP714A1 gene was highly expressed in pencil root, mature leaf, and flower tissues, and its expression was upregulated by GA treatment. We generated the IbCYP714A1 overexpression (OE) Arabidopsis and sweetpotato, which showed similar dwarf phenotype. Additionally, the overexpression of IbCYP714A gene resulted in a significant inhibition of storage root expansion. Further analyses revealed that the levels of bioactive GA (GA₄ and GA₇) were significantly reduced in IbCYP714A1 OE sweetpotato plants. In addition, the levels of GA intermediates such as GA₉, GA₁₅, GA₂₄ and GA₅₃ were significantly reduced in overexpressing plants. This trend of changes in active gibberellin levels coincided with that of plant height changes, further confirming their close association. Meanwhile, the expression of gibberellin metabolism-related genes was decreased in IbCYP714A1 OE sweetpotato plants, which contributed to the reduced gibberellin levels. Taken together, our results conclude that IbCYP714A1 play a critical role in regulating plant height and storage root development by regulating gibberellin signaling pathway in sweet potato.

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甘薯赤霉素失活酶IbCYP714A1的鉴定和功能表征

生物活性赤霉素（GAs）是调控植物生长发育的关键激素，在植物株高等农艺性状中起着重要作用。细胞色素P450基因已成为GA代谢的重要调节因子。在水稻和拟南芥中，ELA1 （CYP714A1）和ELA2 （CYP714A2）编码具有赤霉素失活功能的P450单加氧酶。本研究旨在鉴定甘薯中ga失活的P450基因，并评估其对植株结构和贮藏根发育的功能影响，这是一个关键的产量相关性状。在这项研究中，我们成功地从甘薯品种吉薯26中鉴定了一个细胞色素P450基因IbCYP714A1。IbCYP714A1基因在铅笔根、成熟叶和花组织中高表达，经GA处理后表达上调。我们获得了IbCYP714A1过表达（OE）的拟南芥和甘薯，它们具有相似的矮化表型。此外，IbCYP714A基因的过表达导致贮藏根扩展受到显著抑制。进一步分析表明，IbCYP714A1 OE甘薯植株中具有生物活性的GA （GA4和GA7）水平显著降低。此外，过表达植株中GA中间体如GA9、GA15、GA24和GA53的表达水平显著降低。活性赤霉素水平的变化趋势与株高的变化趋势一致，进一步证实了它们之间的密切联系。同时，赤霉素代谢相关基因在IbCYP714A1 OE甘薯植株中表达减少，导致赤霉素水平降低。综上所述，我们的研究结果表明，IbCYP714A1通过调节赤霉素信号通路在甘薯株高和储根发育中发挥关键作用。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.