Combination of Quercetin and Curcumin modulates NF-κB inflammatory signaling pathway in murine model of esophageal erosive reflux disease erosive reflux disease.

Abstract

Background: This study aimed to investigate whether quercetin, in combination with curcumin, modulates the nuclear factor-kappa B (NF-κB) inflammatory signaling pathway in a murine model of esophageal erosive reflux disease.

Materials and methods: The expression levels of NF-κB and inflammatory factors such as interleukin-1beta (IL-1β), IL-8, and tumor necrosis factor-alpha (TNF-α) were analyzed using Real-Time PCR. Additionally, enzyme-linked immunosorbent assay (ELISA) was employed to validate the Real-Time PCR findings. The western blot study was also conducted to determine the effects on NF-kB p-p65, TNF-α, IL-1β, IL-8.

Results: The results demonstrated that the combination of quercetin and curcumin significantly suppressed the NF-κB inflammatory pathway by reducing the expression of various examined pro-inflammatory cytokines in the murine model of esophageal erosive reflux disease, achieving greater efficacy than when either compound was used individually.

Conclusions: The findings suggest that the synergistic effect of quercetin and curcumin offers a promising approach to mitigating inflammation via modulation of the NF-κB pathway in esophageal erosive reflux disease.