Efficacy of dietary Ceratonia silique and Zingiber offcinale on the immune-antioxidant-signaling pathways, growth, physiological response, and ammonia resistance in Oreochromis niloticus reared under unchanged water.

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mohamed F A Abdel-Aziz, Mona S Azab, Ahmed R Mohamed, Ashraf Y El-Dakar, Dalia S Hamza, Gehad E Elshopakey, Ahmed Shehab, Afaf N Abdel Rahman
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引用次数: 0

Abstract

Prioritizing water management and maintaining its quality for as long as possible, while lowering related stressors, are crucial for sustainable aquaculture. To achieve this equilibrium, enriched aquafeed with natural immunostimulants is essential to success. In this trend, 6 weeks feeding trial was conducted to evaluate the effects of Ceratonia siliqua syrup (CSS) and Zingiber officinale powder (ZOP) in Nile tilapia (Oreochromis niloticus) reared under a zero-water exchange. The immune-antioxidant, growth, physiological responses, and the antioxidant/inflammatory pathways-associated genes as well as ammonia tolerance were evaluated. Fish (weighing 25.85 ± 1.42 g) were randomly housed into six groups (n = 30 fish/group; ten fish/replicate; three replicates/group). The control group was fed a basal diet without any additives. The second (CSS1.25) group was fed a diet supplemented with 1.25% CSS. The third (ZOP0.5) and fourth (ZOP1) groups were fed diets supplemented with 0.5 and 1% ZOP. The fifth (CSS1.25 + ZOP0.5) and sixth (CSS1.25 + ZOP1) groups were fed diets supplemented with 1.25% CSS and 0.5 or 1% ZOP. All treatments were kept without water exchange for 6 weeks. Findings revealed the most notable improvement (P < 0.05) in growth rate (final body weight and specific growth rate) and survival rate in fish fed with dietary ZOP and CSS1.25 + ZOP0.5 diets. Hepato-renal markers (alanine and aspartate aminotransferases, urea, glucose, and cortisol) and lipid peroxides (malonaldehyde) were declined by CSS and/or ZOP diets. Immuno-antioxidants (immunoglobulin M, lysozyme, superoxide dismutase (SOD), and reduced glutathione) were significantly boosted (P < 0.05) in the ZOP1 and CSS1.25 + ZOP0.5 groups. In addition, CSS and/or ZOP diets markedly (P < 0.05) upregulated antioxidant-linked genes (SOD and glutathione peroxidase) and downregulated the stress gene (heat shock protein 70) and pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-alpha). In addition, CSS and/or ZOP diets decreased fish mortality during ammonia stress. The ZOP1 diet was significantly reported of having the best outcomes (P < 0.05) throughout the measured indices. Overall, our findings demonstrate that dietary ZOP and CSS at the optimum doses can improve growth, immune response, and physiological functions of O. niloticus reared in stressful conditions (unchanged water) for the sustainable aquaculture industry.

饲粮中添加白角霉和黑姜对不变水条件下尼罗褐虾免疫-抗氧化信号通路、生长、生理反应和氨氮抗性的影响
优先考虑水管理并尽可能长时间地保持其质量,同时降低相关压力因素,对可持续水产养殖至关重要。为了达到这种平衡,富含天然免疫刺激剂的饲料是成功的关键。在此趋势下,本试验对尼罗罗非鱼(Oreochromis niloticus)进行了为期6周的零换水饲养试验,评价了水藻糖浆(CSS)和生姜粉(ZOP)对尼罗罗非鱼的影响。评估免疫-抗氧化、生长、生理反应、抗氧化/炎症途径相关基因以及氨耐受性。鱼(体重25.85±1.42 g)随机分为6组(n = 30尾/组;十个鱼/复制;三个复制/组)。对照组饲喂不添加任何添加剂的基础饲粮。第二组(CSS1.25)饲喂添加1.25% CSS的饲粮。第三组(ZOP0.5)和第四组(ZOP1)分别饲喂添加0.5%和1% ZOP的饲粮。第5组(CSS1.25 + ZOP0.5)和第6组(CSS1.25 + ZOP1)分别饲喂添加1.25% CSS和0.5或1% ZOP的饲粮。所有处理均不换水6周。结果显示最显著的改善(P
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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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