Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis.

IF 7.5 1区 生物学 Q1 CELL BIOLOGY
Cell reports Pub Date : 2025-06-24 Epub Date: 2025-05-20 DOI:10.1016/j.celrep.2025.115729
Xiaoxiao Liu, Lei Zhang, Zeqi Zhao, Yue Zheng, Yue Ren, Xiaomin Zhao, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Chunlong Li, Chengchao Zheng, Changai Wu
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引用次数: 0

Abstract

Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na+ importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na+ importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na+ accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na+ uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO3--promoted Na+, K+, and Li+ importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na+ import and preventing excessive Na+ accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na+ transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation.

sos2介导的磷酸化对拟南芥NRT1.2/NPF4.6/AIT1非选择性Na+输入能力的调控
虽然阻止钠离子的输入对耐盐性至关重要,但其机制和所涉及的Na+进口商尚不清楚。我们确定NRT1.2/NPF4.6/AIT1为非选择性Na+导入者。在拟南芥中,NRT1.2过表达增加了Na+积累导致的盐敏感性,而NRT1.2突变体表现出相反的结果。无创微测技术检测拟南芥根系NRT1.2对Na+的吸收能力。当NRT1.2在非洲爪蟾卵母细胞中表达时,NRT1.2表现出低ph依赖性和NO3促进的Na+, K+和Li+进口能力,使NRT1.2过表达的植物具有更高的钾和锂耐受性。机械上,盐过度敏感2 (SOS2)在Thr248位点磷酸化NRT1.2,减少Na+的输入,防止Na+的过量积累。长期盐胁迫也下调了NRT1.2转录本。总之,我们的研究结果揭示了NRT1.2在Na+转运中的作用,以及通过sos2介导的NRT1.2在Thr248位点磷酸化的调控途径,这对植物适应盐胁迫至关重要。
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来源期刊
Cell reports
Cell reports CELL BIOLOGY-
CiteScore
13.80
自引率
1.10%
发文量
1305
审稿时长
77 days
期刊介绍: Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.
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