{"title":"Regulation of the non-selective Na<sup>+</sup> importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis.","authors":"Xiaoxiao Liu, Lei Zhang, Zeqi Zhao, Yue Zheng, Yue Ren, Xiaomin Zhao, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Chunlong Li, Chengchao Zheng, Changai Wu","doi":"10.1016/j.celrep.2025.115729","DOIUrl":null,"url":null,"abstract":"<p><p>Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na<sup>+</sup> importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na<sup>+</sup> importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na<sup>+</sup> accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na<sup>+</sup> uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO<sub>3</sub><sup>-</sup>-promoted Na<sup>+</sup>, K<sup>+</sup>, and Li<sup>+</sup> importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na<sup>+</sup> import and preventing excessive Na<sup>+</sup> accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na<sup>+</sup> transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation.</p>","PeriodicalId":9798,"journal":{"name":"Cell reports","volume":"44 6","pages":"115729"},"PeriodicalIF":7.5000,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.celrep.2025.115729","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/20 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na+ importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na+ importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na+ accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na+ uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO3--promoted Na+, K+, and Li+ importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na+ import and preventing excessive Na+ accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na+ transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation.
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